Anti-MUC2 antibody [B306.1] (ab7848)
![Anti-MUC2 antibody [B306.1] (ab7848)](https://www.abcam.com/ps/products/7/ab7848/Images/ab7848-13302-anti-muc2-antibody-b3061-immunohistochemistry.jpg "Anti-MUC2 antibody [B306.1] (ab7848)")
Key features and details
- Mouse monoclonal [B306.1] to MUC2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-MUC2 antibody [B306.1]
See all MUC2 primary antibodies -
Description
Mouse monoclonal [B306.1] to MUC2 -
Host species
Mouse -
Specificity
This antibody reacts with MUC2 gene product, a secretory apomucin found in a variety of normal and neoplastic human glandular epithelia. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic MUC2 tandem repeat consensus sequence.
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Positive control
- Colon carcinoma
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 0.01% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
B306.1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [B306.1] (ab7848)Ab7848 staining human normal colon. Staining is localised to the membrane.
Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
