Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HBEGF/DTR antibody (ab92620)

Key features and details

  • Rabbit polyclonal to HBEGF/DTR
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-HBEGF/DTR antibody
    See all HBEGF/DTR primary antibodies

  • Description

    Rabbit polyclonal to HBEGF/DTR

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human HBEGF/DTR aa 50-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q99075
    (Peptide available as ab104379)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 23 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 23 kDa).
Application notes
Is unsuitable for ICC/IF.

Target

  • Function

    May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

  • Sequence similarities

    Contains 1 EGF-like domain.

  • Post-translational
    modifications

    Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
    O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937).

  • Cellular localization

    Cell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.
  • Information by UniProt

  • Database links

    • Alternative names

      • diphtheria toxin receptor (heparin-binding EGF-like growth factor) antibody
      • diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) antibody
      • Diphtheria toxin receptor antibody
      • DT R antibody
      • DT-R antibody
      • DTR antibody
      • DTS antibody
      • DTSF antibody
      • HB-EGF antibody
      • HBEGF antibody
      • HBEGF_HUMAN antibody
      • HEGFL antibody
      • Heparin binding EGF like growth factor antibody
      • Heparin binding epidermal growth factor antibody
      • Heparin binding epidermal growth factor like growth factor antibody
      • Heparin-binding EGF-like growth factor antibody
      • Proheparin binding EGF like growth factor antibody
      see all

    Images

    • Western blot - Anti-HBEGF/DTR antibody (ab92620)
      Western blot - Anti-HBEGF/DTR antibody (ab92620)
      Anti-HBEGF/DTR antibody (ab92620) at 1 µg/ml + Human stomach tumor tissue lysate (adenocarcinoma) - total protein (ab29678) at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 23 kDa


      Exposure time: 4 minutes


      The band observed at 20 kDa could potentially be a cleaved form of HB EGF due to the presence of a 19 amino acid signal peptide.

    References (8)

    Publishing research using ab92620? Please let us know so that we can cite the reference in this datasheet.

    ab92620 has been referenced in 8 publications.

    • Yi L  et al. CircRNA_30032 promotes renal fibrosis in UUO model mice via miRNA-96-5p/HBEGF/KRAS axis. Aging (Albany NY) 13:12780-12799 (2021). PubMed: 33973871
    • Xiang J  et al. Elevated HB-EGF expression in neural stem cells causes middle age obesity by suppressing Hypocretin/Orexin expression. FASEB J 35:e21345 (2021). PubMed: 33715219
    • Tang H  et al. Forkhead Box Q1 Is Critical to Angiogenesis and Macrophage Recruitment of Colorectal Cancer. Front Oncol 10:564298 (2020). PubMed: 33330033
    • Xu K  et al. Upregulated miR-96-5p inhibits cell proliferation by targeting HBEGF in T-cell acute lymphoblastic leukemia cell line. Folia Histochem Cytobiol 58:219-226 (2020). PubMed: 32893872
    • Tornabene E  et al. Effects of oxygen-glucose deprivation (OGD) on barrier properties and mRNA transcript levels of selected marker proteins in brain endothelial cells/astrocyte co-cultures. PLoS One 14:e0221103 (2019). PubMed: 31425564
    • Zhang Y  et al. Hypothalamic stem cells control ageing speed partly through exosomal miRNAs. Nature 548:52-57 (2017). PubMed: 28746310
    • Gaviglio AL  et al. Heparin-binding epidermal growth factor-like growth factor promotes neuroblastoma differentiation. FASEB J 31:1903-1915 (2017). PubMed: 28174207
    • Altonsy MO  et al. Context-dependent cooperation between nuclear factor ?B (NF-?B) and the glucocorticoid receptor at a TNFAIP3 intronic enhancer: a mechanism to maintain negative feedback control of inflammation. J Biol Chem 289:8231-9 (2014). WB ; Human . PubMed: 24500711

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-HBEGF/DTR antibody (ab92620)
      Immunocytochemistry/ Immunofluorescence - Anti-HBEGF/DTR antibody (ab92620)

      ICC/IF image of ab92620 stained MG63 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab92620 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • Western blot - Anti-HBEGF/DTR antibody (ab92620)
      Western blot - Anti-HBEGF/DTR antibody (ab92620)
      Anti-HBEGF/DTR antibody (ab92620) at 1 µg/ml + Human stomach tumor tissue lysate (adenocarcinoma) - total protein (ab29678) at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 23 kDa


      Exposure time: 4 minutes


      The band observed at 20 kDa could potentially be a cleaved form of HB EGF due to the presence of a 19 amino acid signal peptide.

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