Anti-BSCL2/Seipin antibody (ab106793)
Key features and details
- Rabbit polyclonal to BSCL2/Seipin
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-BSCL2/Seipin antibody
See all BSCL2/Seipin primary antibodies -
Description
Rabbit polyclonal to BSCL2/Seipin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to Human BSCL2/Seipin aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab125933) -
Positive control
- WB: Human, mouse and rat brain; Mouse and rat testis lysate. IHC-P: Human testis tissue.
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General notes
This product was previously labelled as BSCL2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab106793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 44 kDa). IHC-P Use a concentration of 5 µg/ml. Target
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Tissue specificity
Highest expression in brain and testis. -
Involvement in disease
Defects in BSCL2 are the cause of congenital generalized lipodystrophy type 2 (CGL2) [MIM:269700]. Congenital generalized lipodystrophy is an autosomal recessive disorder characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
Defects in BSCL2 are the cause of spastic paraplegia type 17 (SPG17) [MIM:270685]; also known as Silver spastic paraplegia syndrome. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. SPG17 is characterized by prominent amyotrophy of the hand muscles, the presence of mild to severe pyramidal tract signs, and spastic paraplegia. SPG17 is a motor neuron disease overlapping with distal spinal muscular atrophy type 5.
Defects in BSCL2 are a cause of distal hereditary motor neuropathy type 5 (HMN5) [MIM:600794]; also known aS distal hereditary motor neuropathy type V (DSMAV). HMN5 is an autosomal dominant disorder characterized by degeneration of motor nerve fibers, predominantly in limb distal regions. -
Sequence similarities
Belongs to the seipin family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 26580 Human
- Entrez Gene: 14705 Mouse
- Entrez Gene: 361722 Rat
- Omim: 606158 Human
- SwissProt: Q96G97 Human
- SwissProt: Q9Z2E9 Mouse
- SwissProt: Q5FVJ6 Rat
- Unigene: 533709 Human
see all -
Alternative names
- Bernardinelli Seip congenital lipodystrophy 2 antibody
- Bernardinelli Seip congenital lipodystrophy type 2 protein antibody
- Bernardinelli-Seip congenital lipodystrophy type 2 protein antibody
see all
Images
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Western blot - Anti-BSCL2/Seipin antibody (ab106793)All lanes : Anti-BSCL2/Seipin antibody (ab106793) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Testis (Mouse) Tissue Lysate
Lane 5 : Testis (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutesBSCL2/Seipin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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Western blot - Anti-BSCL2/Seipin antibody (ab106793)This image is courtesy of an Abreview submitted by Xavier PrieurAll lanes : Anti-BSCL2/Seipin antibody (ab106793) at 1/1000 dilution
Lane 1 : Wild type mouse white adipose tissue lysate (whole)
Lane 2 : Knockout mouse white adipose tissue lysate (whole)
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BSCL2/Seipin antibody (ab106793)IHC image of BSCL2/Seipin staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab106793, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
References (7)
ab106793 has been referenced in 7 publications.
- Ren Z et al. miR-187-3p inhibitor attenuates cerebral ischemia/reperfusion injury by regulating Seipin-mediated autophagic flux. Int J Mol Med 46:1051-1062 (2020). PubMed: 32705147
- Wang L et al. Seipin deficiency in mice causes loss of dopaminergic neurons via aggregation and phosphorylation of a-synuclein and neuroinflammation. Cell Death Dis 9:440 (2018). PubMed: 29670081
- Mcilroy GD et al. Adipose specific disruption of seipin causes early-onset generalised lipodystrophy and altered fuel utilisation without severe metabolic disease. Mol Metab 10:55-65 (2018). WB ; Mouse . PubMed: 29459250
- Grumati P et al. Full length RTN3 regulates turnover of tubular endoplasmic reticulum via selective autophagy. Elife 6:N/A (2017). WB . PubMed: 28617241
- Zhang Y et al. Echinacoside's nigrostriatal dopaminergic protection against 6-OHDA-Induced endoplasmic reticulum stress through reducing the accumulation of Seipin. J Cell Mol Med 21:3761-3775 (2017). PubMed: 28767194
- Kociucka B et al. Expression of genes involved in lipid droplet formation (BSCL2, SNAP23 and COPA) during porcine in vitro adipogenesis. J Appl Genet 57:505-510 (2016). PubMed: 27108337
- Hsiao CT et al. Clinical and Molecular Characterization of BSCL2 Mutations in a Taiwanese Cohort with Hereditary Neuropathy. PLoS One 11:e0147677 (2016). WB . PubMed: 26815532
Images
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Western blot - Anti-BSCL2/Seipin antibody (ab106793)All lanes : Anti-BSCL2/Seipin antibody (ab106793) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Testis (Mouse) Tissue Lysate
Lane 5 : Testis (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutesBSCL2/Seipin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
-
Western blot - Anti-BSCL2/Seipin antibody (ab106793) This image is courtesy of an Abreview submitted by Xavier PrieurAll lanes : Anti-BSCL2/Seipin antibody (ab106793) at 1/1000 dilution
Lane 1 : Wild type mouse white adipose tissue lysate (whole)
Lane 2 : Knockout mouse white adipose tissue lysate (whole)
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BSCL2/Seipin antibody (ab106793)
IHC image of BSCL2/Seipin staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab106793, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.