Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793)
![Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793)](https://www.abcam.com/ps/products/66/ab66793/Images/ab66793-80865-anti-hb-egf-antibody-4g10-biotin-western-blot.jpg "Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793)")
Key features and details
- Biotin Mouse monoclonal [4G10] to HBEGF/DTR
- Suitable for: IP, ICC/IF, WB
- Reacts with: Human, Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-HBEGF/DTR antibody [4G10]
See all HBEGF/DTR primary antibodies -
Description
Biotin Mouse monoclonal [4G10] to HBEGF/DTR -
Host species
Mouse -
Conjugation
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIP HumanWB MouseHuman
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Immunogen
Recombinant fragment corresponding to Human HBEGF/DTR.
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Epitope
EGF domain -
Positive control
- Vero cell extract carrying human HG-EGF expression vector.
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General notes
Biotin/IgG = 7.5
This product was previously labelled as HB EGF
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Produced in serum-free medium and purified by combination of chromatography. -
Clonality
Monoclonal -
Clone number
4G10 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793)All lanes : Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793) at 1 µg/ml
Lane 1 : Vero cell extract
Lane 2 : Vero cell extract from cells
carrrying human HBEGF/DTR expression vector.
Lane 3 : Vero cell extract from cells carrying
mouse HBEGF/DTR expression vector.
Predicted band size: 23 kDa
Observed band size: 20-28 kDa why is the actual band size different from the predicted?
Additional bands at: 41 kDa. We are unsure as to the identity of these extra bands.
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![Immunocytochemistry/ Immunofluorescence - Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793)](https://www.abcam.com/ps/products/66/ab66793/Images/ab66793-80867-anti-hb-egf-antibody-4g10-biotin-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793)These images shows Vero cells carrying human HG-EGF expression vector (left hand image) and Vero cells carrying mouse HBEGF/DTR expression vector (right hand image). Cells treated with ab66793, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.
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![Immunoprecipitation - Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793)](https://www.abcam.com/ps/products/66/ab66793/Images/ab66793-80866-anti-hb-egf-antibody-4g10-biotin-immunoprecipitation.jpg)
Immunoprecipitation - Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793)All lanes : HBEGF/DTR antibody [4G10](biotin) - Azide free (ab66793) at 1 µg/ml. Lane 1 : Vero cell extract Lane 2 : Vero cell extract from cells carrrying human HBEGF/DTR expression vector. Lane 3 : Vero cell extract from cells carrying mouse HBEGF/DTR expression vector Predicted band size : 23 kDa Observed band size : 20-38 kDa Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands. This image shows Immunoprecipitation of HBEGF/DTR followed by Western blotting/SDS PAGE; the cell surface was biotinylated.
