Anti-HBEGF/DTR antibody [4G10] (ab66792)
![Anti-HBEGF/DTR antibody [4G10] (ab66792)](https://www.abcam.com/ps/products/66/ab66792/Images/ab66792-80864-anti-hb-egf-antibody-4g10-western-blot.jpg "Anti-HBEGF/DTR antibody [4G10] (ab66792)")
Key features and details
- Mouse monoclonal [4G10] to HBEGF/DTR
- Suitable for: IP, WB, ICC/IF
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-HBEGF/DTR antibody [4G10]
See all HBEGF/DTR primary antibodies -
Description
Mouse monoclonal [4G10] to HBEGF/DTR -
Host species
Mouse -
Tested applications
Suitable for: IP, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human HBEGF/DTR.
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Epitope
EGF domain -
Positive control
- Vero cell extract carrrying human HBEGF/DTR expression vector.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Produced in serum-free medium and purified by combination of chromatography -
Clonality
Monoclonal -
Clone number
4G10 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-HBEGF/DTR antibody [4G10] (ab66792)All lanes : Anti-HBEGF/DTR antibody [4G10] (ab66792) at 1 µg/ml
Lane 1 : Vero cell extract
Lane 2 : Vero cell extract from cells
carrrying human HBEGF/DTR expression vector.
Lane 3 : Vero cell extract from cells carrying
mouse HBEGF/DTR expression vector
Predicted band size: 23, 40 kDa
Observed band size: 20-28 kDa why is the actual band size different from the predicted?
Additional bands at: 41 kDa. We are unsure as to the identity of these extra bands.
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![Immunoprecipitation - Anti-HBEGF/DTR antibody [4G10] (ab66792)](https://www.abcam.com/ps/products/66/ab66792/Images/ab66792-80863-anti-hb-egf-antibody-4g10-immunoprecipitation.jpg)
Immunoprecipitation - Anti-HBEGF/DTR antibody [4G10] (ab66792)Lane 1: Vero cell extract, 1μg
Lane 2: Vero cells carrying Human HBEGF/DTR expression vector, 1μg
Lane 3: Vero Cells carrying mouse HBEGF/DTR expression vector, 1μgAb66792 immunoprecipitating HBEGF/DTR in Vero Cells. For western blotting, a HRP conjugated streptavidin was used as the secondary antibody; cell surface was biotinylated.
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![Immunocytochemistry/ Immunofluorescence - Anti-HBEGF/DTR antibody [4G10] (ab66792)](https://www.abcam.com/ps/products/66/ab66792/Images/ab66792-80862-anti-hb-egf-antibody-4g10-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HBEGF/DTR antibody [4G10] (ab66792)These images show Vero cells carrying human HG-EGF expression vector (left hand image) and Vero cells carrying mouse HBEGF/DTR expression vector (right hand image). Cells treated with ab 66792, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.
