Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EGF antibody [EPR19899] - BSA and Azide free (ab251458)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19899] to EGF - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-EGF antibody [EPR19899] - BSA and Azide free
    See all EGF primary antibodies

  • Description

    Rabbit monoclonal [EPR19899] to EGF - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251458 is the carrier-free version of ab206423. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251458 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-EGF antibody [EPR19899] - BSA and Azide free (ab251458)
    Western blot - Anti-EGF antibody [EPR19899] - BSA and Azide free (ab251458)
    All lanes : Anti-EGF antibody [EPR19899] (ab206423) at 1/1000 dilution

    Lane 1 : Recombinant Human Pro-EGF Protein (aa 21-1023) with His-Tag
    Lane 2 : Recombinant Human EGF recombinant protein (aa971-1023)

    Lysates/proteins at 0.015 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 133 kDa



    This data was developed using ab206423, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Western blot - Anti-EGF antibody [EPR19899] - BSA and Azide free (ab251458)
    Western blot - Anti-EGF antibody [EPR19899] - BSA and Azide free (ab251458)
    All lanes : Anti-EGF antibody [EPR19899] (ab206423) at 1/1000 dilution

    Lane 1 : Human EGF recombinant protein (aa971-1023)
    Lane 2 : Mouse EGF recombinant protein (aa977-1029)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 133 kDa
    Observed band size: 6 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This data was developed using ab206423, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-EGF antibody [EPR19899] - BSA and Azide free (ab251458)
    Western blot - Anti-EGF antibody [EPR19899] - BSA and Azide free (ab251458)
    Anti-EGF antibody [EPR19899] (ab206423) at 1/1000 dilution + Mouse salivary gland lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 133 kDa
    Observed band size: 6 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab206423, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 2268351 and 7499272).

  • Anti-EGF antibody [EPR19899] - BSA and Azide free (ab251458)
    Anti-EGF antibody [EPR19899] - BSA and Azide free (ab251458)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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