Mouse Amphiregulin ELISA Kit (ab224882)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 30 pg/ml
- Range: 156.3 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Amphiregulin ELISA Kit
See all Amphiregulin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% RPMI medium 5 4.7% Inter-assay Sample n Mean SD CV% RPMI medium 3 8% -
Sample type
Cell culture supernatant -
Assay type
Sandwich (quantitative) -
Sensitivity
30 pg/ml -
Range
156.3 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 88 85% - 91% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Amphiregulin ELISA Kit (ab224882) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Amphiregulin protein in cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Amphiregulin with 30 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Amphiregulin is a member of the epidermal growth factor family encoded by the AREG gene. Mature Amphiregulin is a 148-amino acid single-pass membrane glycoprotein. Amphiregulin is an autocrine growth factor, a mitogen for several cell types, and is essential for mammary ductal development. Amphiregulin interacts with epidermal growth factor receptor to promote growth of epithelial cells.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml 10X Mouse Amphiregulin Capture Antibody 1 x 600µl 10X Mouse Amphiregulin Detector Antibody 1 x 600µl Mouse Amphiregulin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Bifunctional growth-modulating glycoprotein. Inhibits growth of several human carcinoma cells in culture and stimulates proliferation of human fibroblasts and certain other tumor cells. -
Sequence similarities
Belongs to the amphiregulin family.
Contains 1 EGF-like domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- A REG
- Amphiregulin
- Amphiregulin B
see all -
Database links
- Entrez Gene: 11839 Mouse
- SwissProt: P31955 Mouse
- Unigene: 8039 Mouse
Images
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Other - Mouse Amphiregulin ELISA Kit (ab224882)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of MouseAmphiregulin standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Amphiregulin in Mouse lung cell culture supernatant samples.The concentrations of Amphiregulin were measured in duplicates, interpolated from the Amphiregulin standard curves and corrected for sample dilution. Undiluted samples are as follows: lung cell culture supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Amphiregulin concentration was determined to be 20 ng/mL in neat lung cell culture supernatant.
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Sandwich ELISA - Mouse Amphiregulin ELISA Kit (ab224882)To learn more about the advantages of recombinant antibodies see here.
