Anti-BRD2 (phospho S37) antibody [EPR22114] (ab223255)
![Anti-BRD2 (phospho S37) antibody [EPR22114] (ab223255)](https://www.abcam.com/ps/products/223/ab223255/Images/ab223255-315957-anti-brd2-phospho-s37-antibody-epr22114-western-blot.jpg "Anti-BRD2 (phospho S37) antibody [EPR22114] (ab223255)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22114] to BRD2 (phospho S37)
- Suitable for: Dot blot, WB
- Reacts with: Human
Overview
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Product name
Anti-BRD2 (phospho S37) antibody [EPR22114]
See all BRD2 primary antibodies -
Description
Rabbit monoclonal [EPR22114] to BRD2 (phospho S37) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Dot Blot: BRD2 (phospho S37) peptide (aa31-41) and BRD2 (phospho S37) peptide (aa34-44). WB: Untreated HEK-293 whole cell lysate and HEK-293 treated with 100 nM Calyculin A for 30 minutes, whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22114 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BRD2 (phospho S37) antibody [EPR22114] (ab223255)All lanes : Anti-BRD2 (phospho S37) antibody [EPR22114] (ab223255) at 1/5000 dilution
Lane 1 : Untreated HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : HEK-293 treated with 100 nM Calyculin A for 30 minutes, whole cell lysate
Lane 3 : HEK-293 treated with 100 nM Calyculin A for 30 minutes, whole cell lysate then treated with alkaline phosphatase for 1 hour
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 88 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 92 secondsBlocking/diluting buffer: 5% NFDM/TBST.
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![Dot Blot - Anti-BRD2 (phospho S37) antibody [EPR22114] (ab223255)](https://www.abcam.com/ps/products/223/ab223255/Images/ab223255-315956-anti-brd2-phospho-s37-antibody-epr22114-dot-blot.jpg)
Dot Blot - Anti-BRD2 (phospho S37) antibody [EPR22114] (ab223255)Dot blot analysis of BRD2 (phospho S37) labeled with ab223255 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody was used at 1/1000,000 dilution.
Lane 1: BRD2 (phospho S37) peptide (aa 31-41).
Lane 2: BRD2 (phospho S37) peptide (aa 34-44).
Lane 3: BRD2 non-phospho peptide (aa 31-41).
Exposure time: 10 seconds.
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
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![Anti-BRD2 (phospho S37) antibody [EPR22114] (ab223255)](https://www.abcam.com/ps/products/223/ab223255/Images/ab223255-3-benefits-of-recombinant-antibodies.png)
Anti-BRD2 (phospho S37) antibody [EPR22114] (ab223255)
