Antibodies, Proteins, Kits and Reagents for Life Science

Anti-BRD2 antibody [BL-167-2A2] (ab243865)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BL-167-2A2] to BRD2
Suitable for: WB, IP
Knockout validated
Reacts with: Human

Product name

Anti-BRD2 antibody [BL-167-2A2]
See all BRD2 primary antibodies
Description

Rabbit monoclonal [BL-167-2A2] to BRD2
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human BRD2 aa 675-725. The exact sequence is proprietary. NP_005095.1 and Gene ID 6046.
Database link: P25440
Positive control
- WB: HEK293T, HeLa, SW620, SK, Jurkat, MCF7, HepG2 and A549 cell lysates.
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BL-167-2A2
Isotype

IgG
Research areas
- Epigenetics and Nuclear Signaling
- Bromodomains

Western blot - Anti-BRD2 antibody [BL-167-2A2] (ab243865)

All lanes : Anti-BRD2 antibody [BL-167-2A2] (ab243865) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : BRD2 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Observed band size: 110 kDa
why is the actual band size different from the predicted?

Lanes 1-3: Merged signal (red and green). Green - ab243865 observed at 110 kDa. Red - loading control ab8245 observed at 36 kDa.

ab243865 Anti-BRD2 antibody [BL-167-2A2] was shown to specifically react with BRD2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267265 (knockout cell lysate ab257191) was used. Wild-type and BRD2 knockout samples were subjected to SDS-PAGE. ab243865 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunoprecipitation - Anti-BRD2 antibody [BL-167-2A2] (ab243865)

BRD2 was immunoprecipitated from 0.5 mg HEK-293T whole cell lysate with ab243865 at 10 µl per reaction Western blot was performed on the immunoprecipitate using ab243865 at 1/1000 dilution.
Lane 1: ab243865 IP in HEK-293T cell lysate.
Lane 2: Contol IgG in HEK-293T cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes.
Western blot - Anti-BRD2 antibody [BL-167-2A2] (ab243865)

Western blot analysis using ab243865 at 1/1000 dilution.
Lane 1: HEK-293T whole cell lysate (15 µg).
Lane 2: HeLa whole cell lysate (15 µg).
Lane3: SW620 whole cell lysate (15 µg).
Lane 4: SK whole cell lysate (15 µg).
Lane 5: Jurkat whole cell lysate (15 µg).
Lane 6: MCF7 whole cell lysate (15 µg).
Lane 7: HepG2 whole cell lysate (15 µg).
Lane 8: A549 whole cell lysate (15 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 75 seconds
Anti-BRD2 antibody [BL-167-2A2] (ab243865)

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