HRP Anti-BRD2 antibody [EPR7642] (ab198536)
![HRP Anti-BRD2 antibody [EPR7642] (ab198536)](https://www.abcam.com/ps/products/198/ab198536/Images/ab198536-305224-anti-brd2-antibody-epr7642-hrp-western-blot.jpg "HRP Anti-BRD2 antibody [EPR7642] (ab198536)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR7642] to BRD2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-BRD2 antibody [EPR7642]
See all BRD2 primary antibodies -
Description
HRP Rabbit monoclonal [EPR7642] to BRD2 -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MOLT4, Jurkat, NCCIT and HeLa whole cell lysates.
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General notes
Alternative versions available:
Anti-BRD2 antibody [EPR7642] (ab139690) Knockout validatedThis product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7642 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-BRD2 antibody [EPR7642] (ab198536)All lanes : HRP Anti-BRD2 antibody [EPR7642] (ab198536) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : BRD2 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 88 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesab198536 was shown to specifically react with BRD2 in wild-type HAP1 cells as signal was lost in BRD2 knockout cells. Wild-type and BRD2 knockout samples were subjected to SDS-PAGE. Ab198536 was incubated overnight at 4°C at 1/5000 dilution. Blots were developed with ECL technique.
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![Western blot - HRP Anti-BRD2 antibody [EPR7642] (ab198536)](https://www.abcam.com/ps/products/198/ab198536/Images/ab198536-242582-anti-brd2-antibody-epr7642-hrp-western-blot.jpg)
Western blot - HRP Anti-BRD2 antibody [EPR7642] (ab198536)All lanes : HRP Anti-BRD2 antibody [EPR7642] (ab198536) at 1/5000 dilution
Lane 1 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : NCCIT (Human pluripotent embryonal teratocarcinoma) Whole Cell Lysate
Lane 4 :HeLa whole cell lysate (ab150035)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 88 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198536 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-BRD2 antibody [EPR7642] (ab198536)](https://www.abcam.com/ps/products/198/ab198536/Images/ab198536-3-benefits-of-recombinant-antibodies.png)
HRP Anti-BRD2 antibody [EPR7642] (ab198536)
