Anti-ZNRF1 antibody (ab175125)
Key features and details
- Goat polyclonal to ZNRF1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ZNRF1 antibody -
Description
Goat polyclonal to ZNRF1 -
Host species
Goat -
Specificity
ab175125 is expected to recognize reported isoforms a and b (NP_573469.1 and NP_001162093.1). Reported variants represent identical protein (NP_001162092.1 and NP_573469.1). -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Pig -
Immunogen
Synthetic peptide within Mouse ZNRF1 aa 93-106 (internal sequence) (Cysteine residue). The exact sequence is proprietary.
Sequence:SDSTYAHGNGYQET
Database link: Q91V17 -
Positive control
- Znrf1 transfected N2a cells, Mouse embryo and adult brain lysates and Mouse cerebral cortex tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab175125 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ab175125 (5 µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Immunohistochemical analysis of paraffin embedded Mouse cerebral cortex, labeling ZNRF1 using ab175125 at 10 µg/ml. Streptavidfine-Alexa 488-staining after biotinylated anti-goat secondary.
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All lanes : Anti-ZNRF1 antibody (ab175125) at 1 µg/ml
Lane 1 : Non-transfected N2a cells
Lane 2 : Mouse Znrf1 transfected N2a cells
Lane 3 : Mouse embryo brain lysate
Lane 4 : Mouse adult brain lysate
Developed using the ECL technique.
Predicted band size: 24 kDaPrimary incubation was 1 hour.