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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin E3 Enzymes RING Finger E3 Ligase

Anti-BRD2 antibody (ab245436)

Anti-BRD2 antibody (ab245436)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to BRD2
  • Suitable for: IP, WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-BRD2 antibody
    See all BRD2 primary antibodies
  • Description

    Rabbit polyclonal to BRD2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide within Human BRD2 aa 675-725. The exact sequence is proprietary. NP_005095.1
    Database link: P25440

  • Positive control

    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human ovarian carcinoma tissue. ICC/IF: Asynchronous HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245436 was affinity purified using an epitope specific to BRD2 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • RING Finger E3 Ligase
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Bromodomain containing

Images

  • Western blot - Anti-BRD2 antibody (ab245436)
    Western blot - Anti-BRD2 antibody (ab245436)
    All lanes : Anti-BRD2 antibody (ab245436) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Developed using the ECL technique.

    Predicted band size: 88 kDa


    Exposure time: 3 minutes


    Lysates prepared in NETN buffer.

  • Immunoprecipitation - Anti-BRD2 antibody (ab245436)
    Immunoprecipitation - Anti-BRD2 antibody (ab245436)

    BRD2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245436 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using a different anti-BRD2 antibody at 1/1000 dilution.

    Lane 1: ab245436 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 3 minutes.

    Lysates prepared in NETN buffer.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD2 antibody (ab245436)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD2 antibody (ab245436)

    Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for BRD2 with ab245436 at a 1/200 dilution in immunohistochemical analysis. Detected: DAB.

  • Immunocytochemistry/ Immunofluorescence - Anti-BRD2 antibody (ab245436)
    Immunocytochemistry/ Immunofluorescence - Anti-BRD2 antibody (ab245436)

    Formaldehyde-fixed asynchronous HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling BRD2 using ab245436 at a 1/100 dilution (red) in ICC/IF.

    Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed antibody DyLight®594 conjugated.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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