Protein A/G Sepharose® (ab193262)
Overview
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Product name
Protein A/G Sepharose® -
Product overview
High binding capacity Protein A/G Sepharose (>20 mg IgG/mL). Minimal leaching of ligand. Suitable for column or batch purification of IgG.
Contents:
Supplied as a 50% slurry in 20% Ethanol; > 5mg Protein A/G per mL Sepharose® beads.
Features:
Binding capacity greater than 20 mg/mL of wet gel; high flow rate; low falling off of rProtein A/G; pH stability 2-10.
These beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x g when centrifuging.
Store beads at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
Applications:
Purification of monoclonal and polyclonal antibodies.
Sepharose is a registered trademark of GE Healthcare
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Notes
Protein A/G Sepharose® is prepared by covalently coupling recombinant Protein A/G (contains five Ig-binding regions of protein A and three Ig-binding regions of protein G) to 6% cross-linked Sepharose® beads. The coupling was optimized to give a high binding capacity for IgG. The capacity of IgG binding could be greater than 10 mg of rabbit IgG per ml of wet gel.
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Tested applications
Suitable for: Purificationmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 ml 5 ml 25 ml 100 ml Protein A/G Sepharose® 1 x 1ml 1 x 5ml 1 x 25ml 1 x 100ml -
Research areas
