Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

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Anti-DMC1 antibody [EPR23207-130] (ab245217)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23207-130] to DMC1
Suitable for: WB, IHC-P, IP, IHC-Fr
Reacts with: Mouse, Rat

Product name

Anti-DMC1 antibody [EPR23207-130]
See all DMC1 primary antibodies
Description

Rabbit monoclonal [EPR23207-130] to DMC1
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat
IP	Mouse
WB	Mouse Rat

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: His-tagged mouse DMC1 recombinant protein, Mouse testis, Rat testis lysates. IHC-P: Mouse testis, Rat testis tissues. IP: Mouse testis lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23207-130
Isotype

IgG
Research areas

Western blot - Anti-DMC1 antibody [EPR23207-130] (ab245217)

All lanes : Anti-DMC1 antibody [EPR23207-130] (ab245217) at 1/1000 dilution

Lane 1 : Mouse testis lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Rat testis lysate
Lane 4 : Rat spleen lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 38 kDa
Observed band size: 38 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22156371).

Negative control: Spleen (PMID: 21085687).

Exposure time: 26 seconds
Western blot - Anti-DMC1 antibody [EPR23207-130] (ab245217)

All lanes : Anti-DMC1 antibody [EPR23207-130] (ab245217) at 1/1000 dilution

Lane 1 : His-tagged mouse DMC1 recombinant protein (aa2-340)
Lane 2 : His-tagged mouse Rad51 recombinant protein (aa2-339)

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 38 kDa
Observed band size: 38 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST

No cross-reactivity with mouse Rad51 is observed.

Exposure time: Lane 3 seconds; Lane 2:81 seconds.
Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat testis tissue labeling VSIG4 with ab245217 at 1/100 (6.7 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse testis tissue labeling VSIG4 with ab245217 at 1/100 (6.7 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
Immunoprecipitation - Anti-DMC1 antibody [EPR23207-130] (ab245217)

DMC1 was immunoprecipitated from 0.35 mg Mouse testis lysate 10ug with ab245217 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab245217 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1: Mouse testis lysate 10ug

Lane 2: ab245217 IP in Mouse testis lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245217 in Mouse testis lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling DMC1 with ab245217 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on mouse cerebrum. The section was incubated with ab245217 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling DMC1 with ab245217 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on spermatocytes of rat testis. The section was incubated with ab245217 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling DMC1 with ab245217 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on spermatocytes of mouse testis. The section was incubated with ab245217 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.
Anti-DMC1 antibody [EPR23207-130] (ab245217)

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