Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Glypican 5 antibody [EPR6756(B)] - BSA and Azide free (ab248040)

Western blot - Anti-Glypican 5 antibody [EPR6756(B)] - BSA and Azide free (ab248040)

All lanes : Anti-Glypican 5 antibody [EPR6756(B)] (ab124886) at 1/1000 dilution (Unpurified)

Lane 1 : A549 cell lysate
Lane 2 : Human fetal lung lysate
Lane 3 : HepG2 cell lysate
Lane 4 : C6 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 64 kDa
Observed band size: 63 kDa
why is the actual band size different from the predicted?

This data was developed using ab124886, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 5 antibody [EPR6756(B)] - BSA and Azide free (ab248040)

This data was developed using ab124886, the same antibody clone in a different buffer formulation.
Unpurified ab124886, at 1/250 dilution, staining Glypican 5 in paraffin-embedded Human brain tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 5 antibody [EPR6756(B)] - BSA and Azide free (ab248040)

This data was developed using ab124886, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded human glioma with purified ab124886 at a working dilution of 1/100. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-Glypican 5 antibody [EPR6756(B)] - BSA and Azide free (ab248040)

All lanes : Anti-Glypican 5 antibody [EPR6756(B)] (ab124886) at 1/1000 dilution (purified)

Lane 1 : C6 whole cell lysate
Lane 2 : Mouse spleen tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 64 kDa
Observed band size: 64 kDa

This data was developed using ab124886, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Glypican 5 antibody [EPR6756(B)] - BSA and Azide free (ab248040)

Anti-Glypican 5 antibody [EPR6756(B)] (ab124886) at 1/1000 dilution (purified) + Human fetal lung tissue lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 64 kDa
Observed band size: 64 kDa

This data was developed using ab124886, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Glypican 5 antibody [EPR6756(B)] - BSA and Azide free (ab248040)

Anti-Glypican 5 antibody [EPR6756(B)] (ab124886) at 1/1000 dilution (purified) + A549 whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 64 kDa
Observed band size: 64 kDa

This data was developed using ab124886, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
OI-RD Scanning - Anti-Glypican 5 antibody [EPR6756(B)] - BSA and Azide free (ab248040)

This data was developed using ab124886, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-Glypican 5 antibody [EPR6756(B)] - BSA and Azide free (ab248040)

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Anti-Glypican 5 antibody [EPR6756(B)] - BSA and Azide free (ab248040)

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