Human Neuropilin-1 ELISA Kit (ab227901)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 7 pg/ml
- Range: 37.5 pg/ml - 4800 pg/ml
- Sample type: Cell culture extracts, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Serum, Tissue Culture Media, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Neuropilin-1 ELISA Kit
See all Neuropilin 1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Human Serum 3 1.9% Inter-assay Sample n Mean SD CV% Human Serum 5 3% -
Sample type
Milk, Urine, Serum, Cell culture extracts, Tissue Extracts, Tissue Culture Media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
7 pg/ml -
Range
37.5 pg/ml - 4800 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Milk 89 84% - 97% Urine 83 81% - 85% Serum 110 107% - 113% Cell culture extracts 95 86% - 105% Tissue Extracts 100 94% - 104% Hep Plasma 95 91% - 97% EDTA Plasma 108 106% - 110% Cit plasma 114 111% - 119% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Predicted to work with: Cow
Does not react with:
Non human primates -
Product overview
Human Neuropilin-1 ELISA Kit (ab227901) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Neuropilin-1 protein in cit plasma, edta plasma, hep plasma, milk, serum, tissue culture media, tissue extracts, urine, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Neuropilin-1 with 7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml 10X Human Neuropilin-1 Capture Antibody 1 x 600µl 10X Human Neuropilin-1 Detector Antibody 1 x 600µl Human Neuropilin-1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.
The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity. -
Tissue specificity
The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules. -
Sequence similarities
Belongs to the neuropilin family.
Contains 2 CUB domains.
Contains 2 F5/8 type C domains.
Contains 1 MAM domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- A5 protein
- BDCA4
- BLOOD DENDRITIC CELL ANTIGEN 4
see all -
Database links
- Entrez Gene: 8829 Human
- Omim: 602069 Human
- SwissProt: O14786 Human
- Unigene: 131704 Human
- Unigene: 653996 Human
Images
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Other - Human Neuropilin-1 ELISA Kit (ab227901)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human Neuropilin-1 Standard Curve DataStandard curve comparison between human Neuropilin-1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human Neuropilin-1 standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Example of human Neuropilin-1 standard curve in 1X Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Neuropilin-1 in human serum and plasma samplesThe concentrations of Neuropilin-1 were measured in duplicates, interpolated from the Neuropilin-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:200, plasma (citrate) 1:100, plasma (heparin) 1:100 and plasma (EDTA) 1:100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuropilin-1 concentration was determined to be 330.1 ng/mL in neat serum, 249.9 ng/mL in neat plasma (citrate), 232.5 ng/mL in neat plasma (heparin), and 268.1 ng/mL in neat plasma (EDTA).
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Interpolated concentrations of native Neuropilin-1 in human breast milk and urine samplesThe concentrations of Neuropilin-1 were measured in duplicates, interpolated from the Neuropilin-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: milk 1:50 and urine 1:8. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuropilin-1 concentration was determined to be 24.31 ng/mL in neat breast milk and 10.12 ng/mL in neat urine.
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Interpolated concentrations of native Neuropilin-1 in human HT-29 cell extract based on a 250 µg/mL extract load and human heart tissue extract based on a 125 µg/mL extract loadThe concentrations of Neuropilin-1 were measured in duplicate and interpolated from the Neuropilin-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuropilin-1 concentration was determined to be 2.982 ng/mL in HT-29 cell extract and 2.732 ng/mL in human heart tissue extract.
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Serum from ten individual healthy human female donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuropilin-1 concentration was determined to be 702.5 ng/mL with a range of 533.6 – 937.3 ng/mL.
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Sandwich ELISA - Human Neuropilin-1 ELISA Kit (ab227901)To learn more about the advantages of recombinant antibodies see here.
