Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ku80 antibody [EPR3467] - BSA and Azide free (ab247495)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3467] to Ku80 - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Ku80 antibody [EPR3467] - BSA and Azide free
    See all Ku80 primary antibodies

  • Description

    Rabbit monoclonal [EPR3467] to Ku80 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The immunogen for this antibody has 78.6% homology with mouse and rat ku80, therefore we do not guarantee reactivity in these species.

  • Tested applications

    Suitable for: ICC/IF, IHC-P, Flow Cyt, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247495 is the carrier-free version of ab79391 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247495 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Ku80 antibody [EPR3467] - BSA and Azide free (ab247495)
    Western blot - Anti-Ku80 antibody [EPR3467] - BSA and Azide free (ab247495)
    All lanes : Anti-Ku80 antibody [EPR3467] (ab79391) at 1/2000 dilution

    Lane 1 : A549 cell lysate, treated with FBS
    Lane 2 : HeLa cell lysate
    Lane 3 : U937 (Human histiocytic lymphoma cell line) cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 83 kDa
    Observed band size: 83 kDa



    This data was developed using ab79391, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-Ku80 antibody [EPR3467] - BSA and Azide free (ab247495)
    Flow Cytometry - Anti-Ku80 antibody [EPR3467] - BSA and Azide free (ab247495)
    This data was developed using ab79391, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab79391 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79391, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku80 antibody [EPR3467] - BSA and Azide free (ab247495)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku80 antibody [EPR3467] - BSA and Azide free (ab247495)
    This data was developed using ab79391, the same antibody clone in a different buffer formulation.ab79391 at 1/250 dilution staining Ku80 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-Ku80 antibody [EPR3467] - BSA and Azide free (ab247495)
    Anti-Ku80 antibody [EPR3467] - BSA and Azide free (ab247495)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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