Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Procalcitonin antibody [4A6] - BSA and Azide free (ab255777)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [4A6] to Procalcitonin - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Procalcitonin antibody [4A6] - BSA and Azide free
    See all Procalcitonin primary antibodies

  • Description

    Rat monoclonal [4A6] to Procalcitonin - BSA and Azide free

  • Host species

    Rat

  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human Procalcitonin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P01258

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human thyroid medullary carcinoma and thyroid follicular adenocarcinoma. ICC/IF: TT cell.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255777 is the carrier-free version of ab252821. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Procalcitonin antibody [4A6] - BSA and Azide free (ab255777)
    Immunocytochemistry/ Immunofluorescence - Anti-Procalcitonin antibody [4A6] - BSA and Azide free (ab255777)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized TT cells labelling Procalcitonin with ab252821 at 1/500 dilution, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in TT cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252821).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procalcitonin antibody [4A6] - BSA and Azide free (ab255777)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procalcitonin antibody [4A6] - BSA and Azide free (ab255777)

    Immunohistochemical analysis of paraffin-embedded human thyroid medullary carcinoma tissue labeling Procalcitonin with ab252821 at 1/1000 dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on human thyroid medullary carcinoma. The section was incubated with ab252821 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252821).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procalcitonin antibody [4A6] - BSA and Azide free (ab255777)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procalcitonin antibody [4A6] - BSA and Azide free (ab255777)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Procalcitonin with ab252821 at 1/100 dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Negative control: No staining on human kidney.

    The section was incubated with ab252821 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252821).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procalcitonin antibody [4A6] - BSA and Azide free (ab255777)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procalcitonin antibody [4A6] - BSA and Azide free (ab255777)

    Immunohistochemical analysis of paraffin-embedded human follicular adenocarcinoma of thyroid tissue labeling Procalcitonin with ab252821 at 1/1000 dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on human follicular adenocarcinoma of thyroid. The section was incubated with ab252821 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252821).

  • Anti-Procalcitonin antibody [4A6] - BSA and Azide free (ab255777)
    Anti-Procalcitonin antibody [4A6] - BSA and Azide free (ab255777)

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