All lanes : Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396) at 1 µg/ml

Lanes 1 & 8 : Ladder
Lane 2 : Human liver homogenate at 15 µg
Lane 3 : Human heart homogenate at 15 µg
Lane 4 : Rat liver homogenate at 15 µg
Lane 5 : Rat heart homogenate at 15 µg
Lane 6 : Mouse liver homogenate at 15 µg
Lane 7 : Mouse heart homogenate at 15 µg
Lane 9 : Human fibroblast whole cell lysate at 15 µg
Lane 10 : HeLa whole Cell Lysate at 15 µg
Lane 11 : HepG2 whole cell lysate at 15 µg
Lane 12 : H9C2 (Rat cardiomyoblast cell line) whole Cell Lysate at 15 µg
Lane 13 : H4IIE (Rat hepatoma cell line) whole Cell Lysate at 15 µg
Lane 14 : MEF (Mouse embryonic fibroblast cell line) whole Cell Lysate at 15 µg
Lane 15 : LDH (purified from rabbit skeletal muscle) at 0.015 µg

Secondary
All lanes : Goat polyclonal to Mouse IgG - AP at 1/3000 dilution

Predicted band size: 37 kDa



The high background signal in mouse tissue sample was caused by the direct reaction between the mouse IgG in mouse tissue preps and the goat anti-mouse secondary antibody. LDHA and LDHB are resolved in tissues containing both isoforms.

Flow cytometric analysis of HeLa cells (fixed and permeabilized with methanol) labelling Lactate Dehydrogenase with ab135396 at 1 µg/ml (blue) or an equal amount of an isotype control antibody (red). 1% BSA was used as the blocking reagent for all the blocking steps.

Immunocytochemistry analysis of Paraformaldehyde fixed Hela cells labelling Lactate Dehydrogenase with ab135396 at 1 µg/ml. Detection utilised Alexa 594 goat anti-mouse IgG (H+L) used at 1/1000 dilution.

Immunoprecipitation of Lactate Dehydrogenase from 1mg of Hela Cell Lysate with ab135396 at 1 µg/ml. The gel was stained with Coomassie brilliant blue G.

In-cell ELISA analysis of paraformaldehyde/PBS fixed HeLa cells seeded at 20k cells/well. ab135396 was serially diluted in 1%BSA/PBS and detected with goat anti-mouse dye-conjugated antibody at 1/1000 in 1%BSA/PBS.