Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

50 µl 321 638 ₸

Order now and get it on Tuesday March 09, 2021

Anti-15 Lipoxygenase 1 antibody [7H6] (ab230919)

Key features and details

Mouse monoclonal [7H6] to 15 Lipoxygenase 1
Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
Reacts with: Human, African green monkey
Isotype: IgG2b

Product name

Anti-15 Lipoxygenase 1 antibody [7H6]
See all 15 Lipoxygenase 1 primary antibodies
Description

Mouse monoclonal [7H6] to 15 Lipoxygenase 1
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	African green monkey
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human 15 Lipoxygenase 1. Produced in HEK-293T cells (NP_001131).
Database link: P16050
Positive control
- IHC-P: Human lung and colon tissues. ICC/IF: COS-7 cells. WB: pCMV6-ENTRY ALOX15-transfected HEK-293T cell lysate. Flow Cyt: HEK-293T cells transfected with ALOX15 overexpress plasmid.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

7H6
Isotype

IgG2b
Research areas

Western blot - Anti-15 Lipoxygenase 1 antibody [7H6] (ab230919)

All lanes : Anti-15 Lipoxygenase 1 antibody [7H6] (ab230919) at 1/2000 dilution

Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : pCMV6-ENTRY 15 Lipoxygenase 1-transfected HEK-293T whole cell lysate

Lysates/proteins at 5 µg per lane.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [7H6] (ab230919)

Formalin-fixed, paraffin-embedded human colon tissue stained for 15 Lipoxygenase 1 using ab230919 at 10 μg/ml in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [7H6] (ab230919)

Formalin-fixed, paraffin-embedded human lung tissue stained for 15 Lipoxygenase 1 using ab230919 at 10 μg/ml in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-15 Lipoxygenase 1 antibody [7H6] (ab230919)

pCMV6-ENTRY 15 Lipoxygenase 1-transiently transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for 15 Lipoxygenase 1 (green) using ab230919 at 1/100 dilution in ICC/IF.
Flow Cytometry - Anti-15 Lipoxygenase 1 antibody [7H6] (ab230919)

Flow cytometric analysis of overexpression plasmid-transfected HEK-293T (human breast adenocarcinoma cell line) cells labeling 15 Lipoxygenase 1 with ab230919 at 1/100 dilution (red) compared with an empty vector control-transfected HEK-293T cell line (blue).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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