Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 09, 2021

Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22138] to 15 Lipoxygenase 1
Suitable for: WB, IHC-Fr, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-15 Lipoxygenase 1 antibody [EPR22138]
See all 15 Lipoxygenase 1 primary antibodies
Description

Rabbit monoclonal [EPR22138] to 15 Lipoxygenase 1
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse
IHC-P	Mouse Rat Human Recombinant fragment
WB	Mouse Rat Human Recombinant fragment

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293 transfected with 15 Lipoxygenase 1 (WT) expression vector containing a myc-His-tag®, whole cell lysate; Mouse and rat lung lysates; Human colon lysate. IHC-P: Human colon and bronchus tissues; Mouse colon tissue; Rat bronchus tissue. IHC-Fr: Mouse colon tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22138
Isotype

IgG
Research areas

Immunohistochemistry (Frozen sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling 15 Lipoxygenase 1 with ab244205 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining in eosinophils of mouse colon (PMID:23503644).

The nuclear counter stain is DAPI (blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Western blot - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

All lanes : Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205) at 1/1000 dilution

Lane 1 : Mouse lung lysate
Lane 2 : Rat lung lysate
Lane 3 : Human colon lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 75 kDa
Observed band size: 75 kDa

Exposure time : Lanes 1 and 2: 3 minutes; Lane 3: 15 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID:30118527; PMID:1662607).
Western blot - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

All lanes : Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205) at 1/1000 dilution

Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293 transfected with 15 Lipoxygenase 1 (WT) expression vector containing a myc-His-tag®, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 75 kDa
Observed band size: 75 kDa

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID:30118527; PMID:1662607).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

Immunohistochemical analysis of paraffin-embedded rat bronchus tissue labeling 15 Lipoxygenase 1 with ab244205 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in epithelial cells of rat bronchus (PMID:24036245; PMID:21831839). Counter stained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling 15 Lipoxygenase 1 with ab244205 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in eosinophils of mouse colon (PMID:30118527; PMID:23503644). Counter stained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

Immunohistochemical analysis of paraffin-embedded human bronchus tissue labeling 15 Lipoxygenase 1 with ab244205 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in epithelial cells of human bronchus (PMID:24036245; PMID:21831839). Counter stained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling 15 Lipoxygenase 1 with ab244205 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in eosinophils of human colon (PMID:30118527; PMID:23503644). Counter stained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com