Anti-ECE1 antibody (ab71829)
Key features and details
- Rabbit polyclonal to ECE1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ECE1 antibody -
Description
Rabbit polyclonal to ECE1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Guinea pig, Cow, Xenopus laevis
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Immunogen
Synthetic peptide corresponding to Human ECE1 aa 700 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab87264) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HUVEC MEF1 NIH 3T3
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab71829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 87 kDa). Target
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Relevance
Function: Converts big endothelin-1 to endothelin-1. Tissue specificity: All isoforms are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Isoforms A, B and C are also expressed in placenta, lung, heart, adrenal gland and phaeochromocytoma; isoforms A and C in liver, testis and small intestine; isoform B, C and D in endothelial cells and umbilical vein smooth muscle cells; isoforms C and D in saphenous vein cells, and isoform C in kidney. Disease: Hirschsprung disease cardiac defects and autonomic dysfunction Similarity: Belongs to the peptidase M13 family. -
Cellular localization
Cell Membrane. Single pass type II membrane protein. -
Database links
- Entrez Gene: 281133 Cow
- Entrez Gene: 100719142 Guinea pig
- Entrez Gene: 1889 Human
- Entrez Gene: 230857 Mouse
- Entrez Gene: 94204 Rat
- Omim: 600423 Human
- SwissProt: P42891 Cow
- SwissProt: P97739 Guinea pig
see all -
Alternative names
- ECE 1 antibody
- ECE antibody
- ECE-1 antibody
see all
Images
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Western blot - Anti-ECE1 antibody (ab71829)All lanes : Anti-ECE1 antibody (ab71829) at 1 µg/ml
Lane 1 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
ECE1 contains 10 predicted glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Datasheets and documents
References (2)
ab71829 has been referenced in 2 publications.
- Ghodrati F et al. The prion protein is embedded in a molecular environment that modulates transforming growth factor ß and integrin signaling. Sci Rep 8:8654 (2018). WB ; Human . PubMed: 29872131
- Padilla J et al. TRAF3IP2 mediates high glucose-induced endothelin-1 production as well as endothelin-1-induced inflammation in endothelial cells. Am J Physiol Heart Circ Physiol 314:H52-H64 (2018). PubMed: 28971844
Images
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Western blot - Anti-ECE1 antibody (ab71829)All lanes : Anti-ECE1 antibody (ab71829) at 1 µg/ml
Lane 1 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
ECE1 contains 10 predicted glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
