Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23288-43] to VIP - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-VIP antibody [EPR23288-43] - BSA and Azide free
    See all VIP primary antibodies

  • Description

    Rabbit monoclonal [EPR23288-43] to VIP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details
    Unsuitable for: IP or WB

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human duodenum tissue; Mouse small intestine tissue; Rat small intestine tissue. IHC-Fr: Mouse colon tissue; Rat colon tissue.

  • General notes

    ab273589 is the carrier-free version of ab272726. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23288-43

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Frozen sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589)
    Immunohistochemistry (Frozen sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat colon tissue labeling VIP with ab272726 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on enteric nerve of rat colon (PMID: 26446924) is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (abab272726).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589)

    Immunohistochemical analysis of paraffin-embedded Human duodenum tissue labeling VIP with ab272726 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on enteric nerve of human duodenum. The section was incubated with ab272726 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (abab272726).

  • Immunohistochemistry (Frozen sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589)
    Immunohistochemistry (Frozen sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse colon tissue labeling VIP with ab272726 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on enteric nerve of mouse colon (PMID: 26446924) is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (abab272726).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589)

    Immunohistochemical analysis of paraffin-embedded Rat small intestine tissue labeling VIP with ab272726 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on enteric nerve of rat small intestine (PMID: 7014205). The section was incubated with ab272726 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (abab272726).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589)

    Immunohistochemical analysis of paraffin-embedded Mouse small intestine tissue labeling VIP with ab272726 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on enteric nerve of mouse small intestine. The section was incubated with ab272726 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (abab272726).

  • Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589)
    Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589)

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