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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation

Anti-PRDM4/PFM1 antibody [EPR9432] - BSA and Azide free (ab249281)

Anti-PRDM4/PFM1 antibody [EPR9432] - BSA and Azide free (ab249281)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9432] to PRDM4/PFM1 - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-PRDM4/PFM1 antibody [EPR9432] - BSA and Azide free
    See all PRDM4/PFM1 primary antibodies
  • Description

    Rabbit monoclonal [EPR9432] to PRDM4/PFM1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab249281 is the carrier-free version of ab156867.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9432
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Arginine Methylation

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM4/PFM1 antibody [EPR9432] - BSA and Azide free (ab249281)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM4/PFM1 antibody [EPR9432] - BSA and Azide free (ab249281)

    This data was developed using ab156867, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue labelling PRDM1/PFM1 with ab156867 at 1/500. An ImmunoHistoprobe (Ready to use) HRP polymer for Rabbit IgG was used as the secondary antibody. Tissue was counterstained with Hematoxylin. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Western blot - Anti-PRDM4/PFM1 antibody [EPR9432] - BSA and Azide free (ab249281)
    Western blot - Anti-PRDM4/PFM1 antibody [EPR9432] - BSA and Azide free (ab249281)
    All lanes : Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : PC-3 cell lysate
    Lane 4 : SH-SY5Y cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 88 kDa
    Observed band size: 88 kDa



    This data was developed using ab156867, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PRDM4/PFM1 antibody [EPR9432] - BSA and Azide free (ab249281)
    Western blot - Anti-PRDM4/PFM1 antibody [EPR9432] - BSA and Azide free (ab249281)
    All lanes : Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867) at 1/1000 dilution

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Rat heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H + L) at 1/1000 dilution

    Predicted band size: 88 kDa
    Observed band size: 88 kDa



    This data was developed using ab156867, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM4/PFM1 antibody [EPR9432] - BSA and Azide free (ab249281)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM4/PFM1 antibody [EPR9432] - BSA and Azide free (ab249281)

    This data was developed using ab156867, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue labelling PRDM1/PFM1 with ab156867 at 1/500. An ImmunoHistoprobe (Ready to use) HRP polymer for Rabbit IgG was used as the secondary antibody. Tissue was counterstained with Hematoxylin. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-PRDM4/PFM1 antibody [EPR9432] - BSA and Azide free (ab249281)
    Immunocytochemistry - Anti-PRDM4/PFM1 antibody [EPR9432] - BSA and Azide free (ab249281)
    This data was developed using ab156867, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PRDM4/PFM1 with ab156867 at 1/250. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugtaed goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI.
  • Immunocytochemistry - Anti-PRDM4/PFM1 antibody [EPR9432] - BSA and Azide free (ab249281)
    Immunocytochemistry - Anti-PRDM4/PFM1 antibody [EPR9432] - BSA and Azide free (ab249281)
    This data was developed using ab156867, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of 293 cells labelling PRDM4/PFM1 with ab156867 at 1/250. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugtaed goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI.
  • Anti-PRDM4/PFM1 antibody [EPR9432] - BSA and Azide free (ab249281)
    Anti-PRDM4/PFM1 antibody [EPR9432] - BSA and Azide free (ab249281)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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