Prolactin/PRL overexpression 293T lysate (whole cell) (ab94057)
Overview
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Product name
Prolactin/PRL overexpression 293T lysate (whole cell) -
General notes
ab94057 is a 293T cell transfected lysate in which Human Prolactin has been transiently over-expressed using a pCMV-Prolactin plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details on how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands.
Images
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SDS-PAGE - Prolactin/PRL overexpression 293T lysate (whole cell) (ab94057)ab94057 at 15 µg/lane on an SDS-PAGE gel
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Western blot - Prolactin/PRL overexpression 293T lysate (whole cell) (ab94057)All lanes : Anti-Prolactin/PRL antibody (ab57741) at 1/500 dilution
Lane 1 :Prolactin/PRL overexpression 293T lysate (whole cell) (ab94057)
Lane 2 : 293T non-transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
