Anti-Inhibin beta B antibody (ab69286)
Key features and details
- Rabbit polyclonal to Inhibin beta B
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human, Zebrafish
- Isotype: IgG
Overview
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Product name
Anti-Inhibin beta B antibody
See all Inhibin beta B primary antibodies -
Description
Rabbit polyclonal to Inhibin beta B -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human, Zebrafish
Predicted to work with: Sheep, Chicken, Cow, Cat, Pig, Monkey
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Immunogen
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Positive control
- This antibody gave a postive signal in the following tissue lysates: Human testis; Human pituitary gland; Human adipose tissue. It also gave a positive signal in human testis tissue sections. This antibody gave a positive result in IF in the following formaldehyde fixed cell lines: DU145.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab69286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ICC/IF Use a concentration of 5 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 45 kDa). Target
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Function
Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 396126 Chicken
- Entrez Gene: 530430 Cow
- Entrez Gene: 3625 Human
- Entrez Gene: 397490 Pig
- Entrez Gene: 30275 Zebrafish
- Omim: 147390 Human
- SwissProt: P27093 Chicken
- SwissProt: P42917 Cow
see all -
Alternative names
- Activin beta B chain antibody
- Activin beta-B chain antibody
- INHBB antibody
see all
Images
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Western blot - Anti-Inhibin beta B antibody (ab69286)All lanes : Anti-Inhibin beta B antibody (ab69286) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Brain: pituitary gland (Human) Whole Cell Lysate - adult normal tissue (ab29749)
Lane 3 : Human adipose normal tissue lysate - total protein (ab28980)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa, 22 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
Human Inhibin beta B chain precursor contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Western blot - Anti-Inhibin beta B antibody (ab69286) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin beta B antibody (ab69286)IHC image of ab69286 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab69286, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunocytochemistry/ Immunofluorescence - Anti-Inhibin beta B antibody (ab69286)ICC/IF image of ab69286 stained DU145 cells. The cells were 4% formaldehyde fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab69286 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-Inhibin beta B antibody (ab69286)All lanes : Anti-Inhibin beta B antibody (ab69286) at 2 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Zebrafish heart homogenate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Protocols
Datasheets and documents
References (1)
ab69286 has been referenced in 1 publication.
- Zou G et al. Inhibin B suppresses anoikis resistance and migration through the transforming growth factor-ß signaling pathway in nasopharyngeal carcinoma. Cancer Sci 109:3416-3427 (2018). PubMed: 30151927
Images
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Western blot - Anti-Inhibin beta B antibody (ab69286)All lanes : Anti-Inhibin beta B antibody (ab69286) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Brain: pituitary gland (Human) Whole Cell Lysate - adult normal tissue (ab29749)
Lane 3 : Human adipose normal tissue lysate - total protein (ab28980)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa, 22 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
Human Inhibin beta B chain precursor contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Western blot - Anti-Inhibin beta B antibody (ab69286)
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin beta B antibody (ab69286)IHC image of ab69286 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab69286, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunocytochemistry/ Immunofluorescence - Anti-Inhibin beta B antibody (ab69286)
ICC/IF image of ab69286 stained DU145 cells. The cells were 4% formaldehyde fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab69286 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-Inhibin beta B antibody (ab69286)All lanes : Anti-Inhibin beta B antibody (ab69286) at 2 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Zebrafish heart homogenate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
