Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Wednesday March 10, 2021

Anti-15 Lipoxygenase 1 antibody [EPR22136] (ab242062)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22136] to 15 Lipoxygenase 1
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-15 Lipoxygenase 1 antibody [EPR22136]
See all 15 Lipoxygenase 1 primary antibodies
Description

Rabbit monoclonal [EPR22136] to 15 Lipoxygenase 1
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Recombinant fragment
IHC-P	Human
WB	Human Recombinant fragment

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: 15 Lipoxygenase 1 (WT) transfected HEK-293T whole cell lysate. Human colon lysate. IHC-P: Human bronchia and colon tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22136
Isotype

IgG
Research areas

Western blot - Anti-15 Lipoxygenase 1 antibody [EPR22136] (ab242062)

Anti-15 Lipoxygenase 1 antibody [EPR22136] (ab242062) at 1/1000 dilution + Human colon at 20 µg

Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 75 kDa
Observed band size: 74 kDa
why is the actual band size different from the predicted?

Exposure time: 114 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID:30118527; PMID:1662607).
Western blot - Anti-15 Lipoxygenase 1 antibody [EPR22136] (ab242062)

All lanes : Anti-15 Lipoxygenase 1 antibody [EPR22136] (ab242062) at 1/1000 dilution

Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293T transfected with 15 Lipoxygenase 1 (WT) expression vector containing a myc-His-tag®, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 75 kDa

Exposure time: 37 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID:30118527; PMID:1662607).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22136] (ab242062)

Immunohistochemical analysis of paraffin-embedded human bronchia tissue labeling 15 Lipoxygenase 1 with ab242062 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in epithelial cells of human bronchia (PMID:24036245; PMID:21831839) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22136] (ab242062)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling 15 Lipoxygenase 1 with ab242062 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in eosinophils of human colon (PMID:30118527; PMID:23503644) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-15 Lipoxygenase 1 antibody [EPR22136] (ab242062)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com