Anti-15 Lipoxygenase 1 antibody [EPR22136] - BSA and Azide free (ab243555)
![Anti-15 Lipoxygenase 1 antibody [EPR22136] - BSA and Azide free (ab243555)](https://www.abcam.com/ps/products/243/ab243555/Images/ab243555-2-242062IHC2.jpg "Anti-15 Lipoxygenase 1 antibody [EPR22136] - BSA and Azide free (ab243555)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22136] to 15 Lipoxygenase 1 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-15 Lipoxygenase 1 antibody [EPR22136] - BSA and Azide free
See all 15 Lipoxygenase 1 primary antibodies -
Description
Rabbit monoclonal [EPR22136] to 15 Lipoxygenase 1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 15 Lipoxygenase 1 (WT) transfected HEK-293T whole cell lysate. Human colon lysate. IHC-P: Human bronchia and colon tissue.
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General notes
Ab243555 is the carrier-free version of ab242062. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab243555 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22136 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22136] - BSA and Azide free (ab243555)
Immunohistochemical analysis of paraffin-embedded human bronchia tissue labeling 15 Lipoxygenase 1 with ab242062 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in epithelial cells of human bronchia (PMID:24036245; PMID:21831839) is observed. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242062).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22136] - BSA and Azide free (ab243555)](https://www.abcam.com/ps/products/243/ab243555/Images/ab243555-1-242062IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22136] - BSA and Azide free (ab243555)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling 15 Lipoxygenase 1 with ab242062 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in eosinophils of human colon (PMID:30118527; PMID:23503644) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242062).
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![Anti-15 Lipoxygenase 1 antibody [EPR22136] - BSA and Azide free (ab243555)](https://www.abcam.com/ps/products/243/ab243555/Images/ab243555-5-benefits-of-recombinant-antibodies.png)
Anti-15 Lipoxygenase 1 antibody [EPR22136] - BSA and Azide free (ab243555)
