Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Prolactin/PRL antibody [EPR18018-31] - BSA and Azide free (ab226491)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18018-31] to Prolactin/PRL - BSA and Azide free
Suitable for: WB, IHC-P, IHC-Fr, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-Prolactin/PRL antibody [EPR18018-31] - BSA and Azide free
See all Prolactin/PRL primary antibodies
Description

Rabbit monoclonal [EPR18018-31] to Prolactin/PRL - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, IHC-Fr, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Mouse pituitary tissue.
General notes
ab226491 is the carrier-free version of ab188229.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18018-31
Isotype

IgG
Research areas

Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR18018-31] - BSA and Azide free (ab226491)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse pituitary tissue labeling Prolactin/PRL with ab188229 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Cytoplasmic staining in lactotrophic cells of anterior pituitary gland (PMID: 16468032). The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188229).
Immunoprecipitation - Anti-Prolactin/PRL antibody [EPR18018-31] - BSA and Azide free (ab226491)

Prolactin/PRL was immunoprecipitated from 0.35 mg of mouse pituitary tissue lysate with ab188229 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188229 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Mouse pituitary tissue lysate 10 µg (Input).

Lane 2: ab188229 IP in mouse pituitary tissue lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab188229 in mouse pituitary tissue lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188229).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] - BSA and Azide free (ab226491)

Immunohistochemical analysis of paraffin-embedded rat pituitary tissue labeling Prolactin/PRL with ab188229 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat pituitary (PMID: 24514953). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188229).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] - BSA and Azide free (ab226491)

Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue labeling Prolactin/PRL with ab188229 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse pituitary (PMID: 24514953). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188299).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Prolactin/PRL antibody [EPR18018-31] - BSA and Azide free (ab226491)

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