Anti-Relaxin 3 receptor 1 antibody (ab72914)
Key features and details
- Rabbit polyclonal to Relaxin 3 receptor 1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Relaxin 3 receptor 1 antibody
See all Relaxin 3 receptor 1 primary antibodies -
Description
Rabbit polyclonal to Relaxin 3 receptor 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Relaxin 3 receptor 1 aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab89370) -
Positive control
- This antibody gave a positive signal in the SKNSH whole cell lysate and the following tissue lysates: Human brain; Human brain membrane; Human spinal cord; Human kidney.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).Target
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Function
Receptor for RNL3/relaxin-3. Binding of the ligand inhibit cAMP accumulation. -
Tissue specificity
Expressed predominantly in brain regions. Highest expression in substantia nigra and pituitary, followed by hippocampus, spinal cord, amygdala, caudate nucleus and corpus callosum, quite low level in cerebellum. In peripheral tissues, relatively high levels in adrenal glands, low levels in pancreas, salivary gland, placenta, mammary gland and testis. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 51289 Human
- Entrez Gene: 239336 Mouse
- Entrez Gene: 294807 Rat
- Omim: 609445 Human
- SwissProt: Q9NSD7 Human
- SwissProt: Q8BGE9 Mouse
- Unigene: 170146 Human
- Unigene: 209312 Mouse
see all -
Alternative names
- G protein coupled receptor GPCR135 antibody
- G protein coupled receptor SALPR antibody
- G protein-coupled receptor SALPR antibody
see all
Images
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Western blot - Anti-Relaxin 3 receptor 1 antibody (ab72914)All lanes : Anti-Relaxin 3 receptor 1 antibody (ab72914) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human brain normal tissue lysate - membrane extract (ab29456)
Lane 3 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 4 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lane 5 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 37 kDa, 49 kDa (possible glycosylated form). We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Relaxin-3 receptor 1 (RXFP3)has multiple glycosylation sites (SwissProt) as can be seen by the characteristic banding observed at 49 kDa.
Datasheets and documents
References (0)
ab72914 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Relaxin 3 receptor 1 antibody (ab72914)All lanes : Anti-Relaxin 3 receptor 1 antibody (ab72914) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human brain normal tissue lysate - membrane extract (ab29456)
Lane 3 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 4 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lane 5 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 37 kDa, 49 kDa (possible glycosylated form). We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Relaxin-3 receptor 1 (RXFP3)has multiple glycosylation sites (SwissProt) as can be seen by the characteristic banding observed at 49 kDa.
