TripleStain IHC Kit: M&M&R on human tissue (DAB, Red/AP & DAB/Ni) (ab183287)
Overview
-
Product name
TripleStain IHC Kit: M&M&R on human tissue (DAB, Red/AP & DAB/Ni) -
Assay type
Polymer Multiplex -
Product overview
The TripleStain IHC Kit (ab183287) is designed to use with two mouse primary antibodies and one rabbit primary antibody to detect three distinct antigens on a single human tissue or cell samples. This kit has been tested in paraffin-embedded tissues, however, it can also be used on frozen specimens and freshly prepared monolayer cell smears.
Triple staining is a common method used in immunohistochemistry for the detection of three distinct antigens in a single tissue. Abcam’s TripleStain IHC Kit supplies polymer enzyme conjugates: polymer-HRP anti-mouse IgG, polymer-AP anti-mouse IgG, and polymer-HRP anti-rabbit IgG with three substrates/chromogens; DAB (brown), DAB-Ni (black), and Permanent Red (Red). The TripleStain Kit is a non-biotin system avoiding non-specific background due to endogenous biotin binding. A Primer step is used to increase specificity of antibody staining. This kit has been optimized to have no cross detection when detecting two primary antibodies from the same host species using a unique blocking system. Simplified steps allow users to complete triple staining within 5 hours (without antigen retrieval) or 6 hours (with antigen retrieval). The well tested protocol provides the user with the ability to permanently mount slides with a coverslip.
-
Tested applications
Suitable for: IHC-Fr, IHC-Pmore details
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 24 ml 72 ml 240 ml Antibody Blocker (40x) 2 x 15ml 1 x 50ml 1 x 100ml Aqueous Mounting Medium 1 x 15ml 2 x 18ml 1 x 120ml Blocker A 1 x 12ml 2 x 18ml 1 x 120ml Blocker B 1 x 12ml 2 x 18ml 1 x 120ml DAB Chromogen (20x) 1 x 1.5ml 1 x 2ml 1 x 6ml DAB Substrate 1 x 15ml 2 x 18ml 1 x 120ml DAB-Ni Substrate (20x) 1 x 1ml 1 x 2ml 1 x 6ml Hydrogen Peroxide (20x) 1 x 1ml 1 x 2ml 1 x 6ml Mouse AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Mouse HRP Polymer 1 x 12ml 2 x 18ml 1 x 120ml Nickel Solution (7x) 1 x 3ml 1 x 6ml 1 x 18ml Permanent Red Activator (5x) 1 x 3ml 1 x 7.2ml 2 x 12ml Permanent Red Chromogen (100x) 1 x 150µl 1 x 360µl 1 x 1.2ml Permanent Red Substrate 1 x 15ml 2 x 18ml 1 x 120ml Rabbit HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml -
Research areas
Images
-
Immunohistochemistry (Paraffin-embedded sections)
Immunohistochemical analysis of paraffin embedded human colon cancer tissue. Mouse anti-PCNA (black), rabbit anti-CEA (brown), mouse anti-smooth muscle Actin (red).
