Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human Colipase ELISA Kit (ab230937)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 3 pg/ml
  • Range: 5 pg/ml - 300 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Homogenate, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Colipase ELISA Kit
    See all CLPS kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    EDTA plasma 5 4.9%
    Inter-assay
    Sample n Mean SD CV%
    EDTA plasma 3 11.1%

  • Sample type

    Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma, Tissue Homogenate

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    3 pg/ml

  • Range

    5 pg/ml - 300 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 103 102% - 104%
    Urine 90 86% - 94%
    Serum 98 97% - 99%
    Hep Plasma 108 101% - 121%
    EDTA Plasma 111 104% - 124%
    Cit plasma 111 104% - 120%
    Tissue Homogenate 102 100% - 103%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human
    Does not react with: Cow

  • Product overview

    Human Colipase ELISA Kit (ab230937) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Colipase protein in cit plasma, edta plasma, hep plasma, serum, tissue homogenate, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Colipase with 3 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Human Colipase ELISA Kit (ab230937)
    Other - Human Colipase ELISA Kit (ab230937)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human Colipase standard curve in Sample Diluent NS
    Example of human Colipase standard curve in Sample Diluent NS

    Background-subtracted data values (mean +/- SD) are graphed.

  • Example of human Colipase standard curve in 1X Cell Extraction Buffer PTR
    Example of human Colipase standard curve in 1X Cell Extraction Buffer PTR

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native Colipase in human serum, plasma, and urine samples
    Interpolated concentrations of native Colipase in human serum, plasma, and urine samples

    The concentrations of Colipase were measured in duplicates, interpolated from the Colipase standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 3.1%, plasma (heparin) 10%, plasma (EDTA) 6.3%, and urine 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Colipase concentration was determined to be 2684 pg/mL in serum, 5049 pg/mL in plasma (citrate), 2526 pg/ml in plasma (heparin), 3666 pg/ml in plasma (EDTA), and 613 pg/mL in urine.

  • Interpolated concentrations of native Colipase in human pancreas homogenate extract based on a 0.04 µg/mL extract load
    Interpolated concentrations of native Colipase in human pancreas homogenate extract based on a 0.04 µg/mL extract load

    The concentrations of Colipase were measured in duplicate and interpolated from the Colipase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Colipase concentration was determined to be 243 pg/mL in human pancreas homogenate extract at this loading.

  • Serum from ten individual healthy human male donors was measured in duplicate
    Serum from ten individual healthy human male donors was measured in duplicate

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Colipase concentration was determined to be 3348 pg/mL with a range of 1229 – 5295 pg/mL.

  • Sandwich ELISA - Human Colipase ELISA Kit (ab230937)
    Sandwich ELISA - Human Colipase ELISA Kit (ab230937)
    To learn more about the advantages of recombinant antibodies see here.

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