Antibodies, Proteins, Kits and Reagents for Life Science

Recombinant human PD1 protein (Fc Chimera Active) (ab221398)

Key features and details

Expression system: HEK 293 cells
Purity: > 95% SDS-PAGE
Endotoxin level:
Active: Yes
Suitable for: SDS-PAGE, Functional Studies, HPLC

Stability and Storage

Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.

pH: 7.40
Constituents: PBS, 5% Trehalose

This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitution

Reconstitute with sterile deionized water to a concentration of 400 µg/ml.

Functional Studies - Recombinant human PD1 protein (Fc Chimera Active) (ab221398)

Human PD-1, Fc Tag, low endotoxin (HPLC-verified) captured on CM5 chip via anti-human IgG Fc antibody, can bind Human PD-L1, His Tag (HPLC-verified) with an affinity constant of 3.6 µM as determined in a SPR assay (Biacore T200).
Functional Studies - Recombinant human PD1 protein (Fc Chimera Active) (ab221398)

The purity of Human PD-1, Fc Tag, low endotoxin (HPLC-verified) was greater than 90% as determined by SEC-HPLC.
Functional Studies - Recombinant human PD1 protein (Fc Chimera Active) (ab221398)

Flow Cytometry assay shows that Human PD-1, Fc Tag, low endotoxin (HPLC-verified) can bind to 293 cell overexpressing human PD-L1. The concentration of PD-1 used is 1 µg/mL.
Functional Studies - Recombinant human PD1 protein (Fc Chimera Active) (ab221398)

Immobilized Biotinylated Human PD-L2, Avitag,His Tag (recommended for biopanning) at 1 µg/mL (100 µL/well) on streptavidin precoated (0.2 µg/well) plate, can bind Human PD-1, Fc Tag, low endotoxin (HPLC-verified) with a linear range of 0.1-1 ng/mL.
Functional Studies - Recombinant human PD1 protein (Fc Chimera Active) (ab221398)

Immobilized Human PD-1, Fc Tag, low endotoxin (HPLC-verified) at 2 µg/mL (100 µL/well) can bind Biotinylated Human PD-L2, Fc,Avitag with a linear range of 0.5-16 ng/mL.
Functional Studies - Recombinant human PD1 protein (Fc Chimera Active) (ab221398)

Immobilized Biotinylated Human PD-L1, Avitag,His Tag (recommended for biopanning) at 1 µg/mL (100 µL/well) on streptavidin precoated (0.2 µg/well) plate, can bind Human PD-1, Fc Tag, low endotoxin (HPLC-verified) with a linear range of 0.1-3 ng/mL.
Functional Studies - Recombinant human PD1 protein (Fc Chimera Active) (ab221398)

Loaded Human PD-1, Fc Tag, low endotoxin (HPLC-verified) on Protein A Biosensor, can bind Human PD-L1, His Tag (HPLC & BLI verified) with an affinity constant of 5.3µM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
SDS-PAGE - Recombinant human PD1 protein (Fc Chimera Active) (ab221398)

SDS-PAGE using ab221398 under reducing (R) condition. The gel was stained overnight with Coomassie Blue.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com