Recombinant Human PD1 protein (ab233685)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant Human PD1 protein
See all PD1 proteins and peptides -
Purity
> 90 % SDS-PAGE.
>90% purity as measured by gel filtration. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
LDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSN QTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCG AISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQ -
Predicted molecular weight
20 kDa including tags -
Amino acids
25 to 167 -
Additional sequence information
C-terminal FLAG-Avi-His-tags (NM_005018).
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), Phosphate Buffer
Images
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SDS-PAGE - Recombinant Human PD1 protein (ab233685)
4-20%% SDS-PAGE analysis of 3 µg ab233685, stained with Coomassie.
This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
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Size Exclusion Chromatography - Recombinant Human PD1 protein (ab233685)Gel Filtration curve.
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Functional Studies - Recombinant Human PD1 protein (ab233685)10 nM PD-L1 was Biotin labeled then mixed with varying amounts of PD-1, FLAG-tag in PD-1 Assay Buffer for 1 hr at RT. FLAG-acceptor beads were added and incubated for 30 min at RT followed by streptavidin-conjugated donor beads for 30 min at RT. Alpha counts were read.
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Functional Studies - Recombinant Human PD1 protein (ab233685)10 nM PD-L1, was Biotin labeled then mixed with varying amounts of PD-1, FLAG-tag in PD-1 Assay Buffer for 1 hr at RT. Nickel-acceptor beads were added and incubated for 30 min at RT followed by streptavidin-conjugated donor beads for 30 min at RT. Alpha counts were read.
