Recombinant human PD1 protein (Active) (ab174035)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: HPLC, SDS-PAGE, Functional Studies, ELISA
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C. Please see notes section.
pH: 7.40
Constituents: PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein with 500µL sterile deionized water to a stock solution of 200 µg/mL.
Images
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Functional Studies - Recombinant human PD1 protein (ab174035)
Immobilized ab174035 at 0.2μg/mL (100 μL/well) binds Human PD-L1, Fc Tag.
Linear range of 0.31-1.25 μg/mL (QC tested).
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Functional Studies - Recombinant human PD1 protein (Active) (ab174035)Loaded Human PD-1 (His Tag) (HPLC verified) on HIS1K Biosensor can bind Human PD-L2 (Fc Tag) (HPLC verified) with an affinity constant of 16.3 nM as determined in bli assay (ForteBio Octet Red96e) (QC tested). -
Functional Studies - Recombinant human PD1 protein (ab174035)Immobilized ab174035 at 0.2 μg/mL ( 100 μl/well ) binds Human PD-L2, Fc Tag.
Linear range of 0.16-2.5 μg/mL (QC tested).
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Functional Studies - Recombinant human PD1 protein (Active) (ab174035)Loaded Human PD-1 (His Tag) (HPLC verified) on HIS1K Biosensor can bind Human PD-L1 (Fc Tag) (HPLC verified) with an affinity constant of 38.9 nM as determined in bli assay (ForteBio Octet Red96e) (QC tested).
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Functional Studies - Recombinant human PD1 protein (ab174035)Immobilized ab174035 at 2 μg/mL (100 μL/well) binds Human PD-L2, Mouse IgG1 Fc Tag.
Linear range of 10-156 ng/mL (Routinely tested).
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Functional Studies - Recombinant human PD1 protein (ab174035)Immobilized ab174035 at 2 μg/mL (100 μL/well) binds Nivolumab.
Linear range of 0.1-3 ng/mL (Routinely tested).
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Functional Studies - Recombinant human PD1 protein (ab174035)Opdivo(Nivolumab), captured on CM5 chip via anti-human IgG Fc antibodies surface, binds ab174035 with an affinity constant of 4.94 nM, as determined in an SPR assay (Biacore T200) (Routinely tested).
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SDS-PAGE - Recombinant human PD1 protein (ab174035)Reduced ab174035 on SDS-PAGE, stained overnight with Coomassie Blue.
The purity of the protein is greater than 95%.
The protein migrates as 31-44kDa under reducing conditions, due to glycosylation.
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HPLC - Recombinant human PD1 protein (ab174035)The purity of ab174035 was greater than 90%, as determined by SEC-HPLC.
