Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Topoisomerase I antibody [EPR5376(2)] (ab131166)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5376(2)] to Topoisomerase I
Suitable for: WB, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-Topoisomerase I antibody [EPR5376(2)]
See all Topoisomerase I primary antibodies
Description

Rabbit monoclonal [EPR5376(2)] to Topoisomerase I
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- MCF7, Jurkat, SW480, K562, Ramos, RAW264.7 and PC12 cell lysates; MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 4.67 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR5376(2)
Isotype

IgG
Research areas

Western blot - Anti-Topoisomerase I antibody [EPR5376(2)] (ab131166)

All lanes : Anti-Topoisomerase I antibody [EPR5376(2)] (ab131166) at 1/1000 dilution

Lane 1 : MCF7 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : SW480 cell lysate
Lane 4 : K562 cell lysate
Lane 5 : Ramos cell lysate
Lane 6 : RAW264.7 cell lysate
Lane 7 : PC12 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 91 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Topoisomerase I antibody [EPR5376(2)] (ab131166)

Immunocytochemistry/Immunofluorescence analysis of HaCaT (Human keratinocyte cell line) labelling Topoisomerase I with purified ab131166 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
Immunocytochemistry/ Immunofluorescence - Anti-Topoisomerase I antibody [EPR5376(2)] (ab131166)

Immunofluorescent analysis of MCF7 cells labelling Topoisomerase I with ab131166 at 1/250 dilution.
OI-RD Scanning - Anti-Topoisomerase I antibody [EPR5376(2)] (ab131166)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-Topoisomerase I antibody [EPR5376(2)] (ab131166)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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