Anti-Topoisomerase I antibody (ab245432)
Key features and details
- Rabbit polyclonal to Topoisomerase I
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Topoisomerase I antibody
See all Topoisomerase I primary antibodies -
Description
Rabbit polyclonal to Topoisomerase I -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseHumanWB Human
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Immunogen
Synthetic peptide within Human Topoisomerase I aa 715-765. The exact sequence is proprietary. NP_003277.1
Database link: P11387 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: Topoisomerase I IP in NIH/3T3 and HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245432 was affinity purified using an epitope specific to Topoisomerase I immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Topoisomerase I antibody (ab245432)All lanes : Anti-Topoisomerase I antibody (ab245432) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Exposure time: 30 secondsPrepared using NETN lysis buffer.
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Immunoprecipitation - Anti-Topoisomerase I antibody (ab245432)Topoisomerase I was immunoprecipitated from NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245432 used for WB 0.1 µg/ml.
Chemiluminescence detection: 30 seconds.
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Immunoprecipitation - Anti-Topoisomerase I antibody (ab245432)Topoisomerase I was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245432 used for IP at 6 µg per reaction. For WB 1 µg/ml.
Lane 1: ab245432 (Lot 2) IP in HeLa whole cell lysate.
Lane 2: ab245432 (Lot 3) IP in HeLa whole cell lysate.
Lane 3: Control IgG in HeLa whole cell lysate.
Chemiluminescence detection: 3 minutes.
