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Anti-Topoisomerase I antibody [EPR5376(2)] - BSA and Azide free (ab240047)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5376(2)] to Topoisomerase I - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Topoisomerase I antibody [EPR5376(2)] - BSA and Azide free
    See all Topoisomerase I primary antibodies

  • Description

    Rabbit monoclonal [EPR5376(2)] to Topoisomerase I - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab240047 is the carrier-free version of ab131166. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240047 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry - Anti-Topoisomerase I antibody [EPR5376(2)] - BSA and Azide free (ab240047)
    Immunocytochemistry - Anti-Topoisomerase I antibody [EPR5376(2)] - BSA and Azide free (ab240047)

    Immunocytochemistry/Immunofluorescence analysis of HaCaT (Human keratinocyte cell line) labelling Topoisomerase I with purified ab131166 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131166).

  • Immunocytochemistry - Anti-Topoisomerase I antibody [EPR5376(2)] - BSA and Azide free (ab240047)
    Immunocytochemistry - Anti-Topoisomerase I antibody [EPR5376(2)] - BSA and Azide free (ab240047)

    Clone EPR5376(2) (ab240047) has been successfully conjugated by Abcam. This image was generated using Anti-Topoisomerase I antibody [EPR5376(2)] (Alexa Fluor® 488). Please refer to ab223421 for protocol details.

    ab223421 staining Topoisomerase I in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223421 at 1/100 dilution (shown in Green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in MCF7 cells fixed with 100% methanol (5 min).

  • Immunocytochemistry - Anti-Topoisomerase I antibody [EPR5376(2)] - BSA and Azide free (ab240047)
    Immunocytochemistry - Anti-Topoisomerase I antibody [EPR5376(2)] - BSA and Azide free (ab240047)

    Clone EPR5376(2) (ab240047) has been successfully conjugated by Abcam. This image was generated using Anti-Topoisomerase I antibody [EPR5376(2)] (Alexa Fluor® 647). Please refer to ab223422 for protocol details.

    ab223422 staining Topoisomerase I in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223422 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in MCF7 cells fixed with 100% methanol (5 min).

  • Immunocytochemistry - Anti-Topoisomerase I antibody [EPR5376(2)] - BSA and Azide free (ab240047)
    Immunocytochemistry - Anti-Topoisomerase I antibody [EPR5376(2)] - BSA and Azide free (ab240047)
    Immunofluorescent analysis of MCF7 cells labelling Topoisomerase I with ab131166 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131166).

  • OI-RD Scanning - Anti-Topoisomerase I antibody [EPR5376(2)] - BSA and Azide free (ab240047)
    OI-RD Scanning - Anti-Topoisomerase I antibody [EPR5376(2)] - BSA and Azide free (ab240047)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131166).

  • Anti-Topoisomerase I antibody [EPR5376(2)] - BSA and Azide free (ab240047)
    Anti-Topoisomerase I antibody [EPR5376(2)] - BSA and Azide free (ab240047)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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