Anti-Topoisomerase I antibody [23B11] (ab200869)
Key features and details
- Mouse monoclonal [23B11] to Topoisomerase I
- Suitable for: WB
- Reacts with: Mouse, Rat, Dog, Human
- Isotype: IgG1
Overview
-
Product name
Anti-Topoisomerase I antibody [23B11]
See all Topoisomerase I primary antibodies -
Description
Mouse monoclonal [23B11] to Topoisomerase I -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Dog, Human
Predicted to work with: Xenopus laevis, Chinese hamster
-
Immunogen
Synthetic peptide corresponding to Human Topoisomerase I aa 650-750 conjugated to keyhole limpet haemocyanin.
Database link: P11387 -
Positive control
- Whole cell lysates of serum starved HeLa, HepG2, HEK293, SH-SY5Y, MDCK PC12, CMT 93, Neuro 2A and 3T3 tumor cells.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.09% Sodium azide
Constituent: PBS
With PEG and sucrose. -
Concentration information loading... -
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
23B11 -
Isotype
IgG1 -
Research areas
Images
-
All lanes : Anti-Topoisomerase I antibody [23B11] (ab200869) at 0.5 µg/ml
Lane 1 : Whole cell lysate of serum starved HeLa cells
Lane 2 : Whole cell lysate of serum starved HepG2 cells
Lane 3 : Whole cell lysate of serum starved HEK293 cells
Lane 4 : Whole cell lysate of serum starved SH-SY5Y cells
Lane 5 : Whole cell lysate of serum starved MDCK cells
Lane 6 : Whole cell lysate of serum starved PC12 cells
Lane 7 : Whole cell lysate of serum starved CMT 93 cells
Lane 8 : Whole cell lysate of serum starved Neuro 2A cells
Lane 9 : Whole cell lysate of serum starved 3T3 tumor cells
Lysates/proteins at 20000 cells per lane.
Developed using the ECL technique.
Predicted band size: 91 kDa
Exposure time: 30 secondsPVDF membrane.

