Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22524-95] to Tau - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Tau antibody [EPR22524-95] - BSA and Azide free
    See all Tau primary antibodies

  • Description

    Rabbit monoclonal [EPR22524-95] to Tau - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human and mouse brain lysate. IHC-P: Mouse cerebral cortex and hippocampus tissue. Human cerebral cortex tissue. IHC-Fr: Mouse cerebral cortex tissue. IP: Human brain lysate.

  • General notes

    Ab255271 is the carrier-free version of ab254256. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255271 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)
    Immunoprecipitation - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)

    Tau was immunoprecipitated from 0.35 mg human brain lysate with ab254256 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab254256 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

    Lane 1: Human brain lysate 10 µg (Input).
    Lane 2: ab254256 IP in human brain lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254256 in human brain lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254256).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Tau with ab254256 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse hippocampus (PMID: 22961084) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254256 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254256).

  • Immunohistochemistry (Frozen sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)
    Immunohistochemistry (Frozen sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebral cortex tissue labeling Tau with ab254256 at 1/100 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on mouse cerebral cortex (PMID: 22961084) is observed. Counterstained with DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254256).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling Tau with ab254256 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebral cortex (PMID: 22961084) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254256 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254256).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)

    Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling Tau with ab254256 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebral cortex (PMID: 22961084) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254256 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254256).

  • Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)
    Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)

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