Mouse Pin1 ELISA Kit (ab209879)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 11.2 pg/ml
- Range: 46.9 pg/ml - 3000 pg/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Pin1 ELISA Kit
See all Pin1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% cell extract 8 3.7% Inter-assay Sample n Mean SD CV% cell extract 3 3.5% -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
11.2 pg/ml -
Range
46.9 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 105 94% - 111% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Pin1 ELISA Kit (ab209879) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Pin1 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Pin1 with 11.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Pin1 is a peptidyl-prolyl cis/trans isomerase, isomerizing specific phosphorylated Ser/Thr-Pro motifs present in some proteins. Pin1 is involved in the regulation of mitosis, and it can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability, and cell transformation. Upregulation of Pin1 is associated with certain cancers, while downregulation of Pin1 has been implicated in Alzheimer’s disease.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Pin1 Capture Antibody 1 x 600µl 10X Mouse Pin1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse Pin1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations. -
Sequence similarities
Contains 1 PpiC domain.
Contains 1 WW domain. -
Domain
The WW domain is required for the interaction with STIL and KIF20B. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- DOD
- DODO, Drosophila, homolog of
- FLJ40239
see all -
Database links
- Entrez Gene: 23988 Mouse
- SwissProt: Q9QUR7 Mouse
- Unigene: 7906 Mouse
Images
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Other - Mouse Pin1 ELISA Kit (ab209879)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Pin1 standard curve in 1X Cell Extraction Buffer PTR.The Pin1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Pin1 in mouse NIH-3T3 extract based on a 35 µg/mL extract load and mouse brain extract based on a 50 µg/mL extract load.The concentrations of Pin1 were measured in duplicate and interpolated from the Pin1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pin1 concentration was determined to be 906 pg/mL in NIH-3T3 extract and 1,803 pg/mL in mouse brain extract.
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This assay detects human and rat PP2A-alpha.The concentrations of PP2A-alpha in PC12 and HeLa extract were measured in duplicate, interpolated from the mouse PP2A-alpha standard curves and corrected for sample dilution. Undiluted samples are PC12 extract based on a 25 μg/mL extract load and HeLa extract based on a 12.5 μg/mL extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PP2A-alpha concentration was determined to be 1,763 pg/mL in PC12 extract and 763 pg/mL in HeLa extract.
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Sandwich ELISA - Mouse Pin1 ELISA Kit (ab209879)To learn more about the advantages of recombinant antibodies see here.
