Anti-Prolactin/PRL antibody [EPR19372] - BSA and Azide free (ab250714)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19372] to Prolactin/PRL - BSA and Azide free
- Suitable for: IP, IHC-Fr, WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-Prolactin/PRL antibody [EPR19372] - BSA and Azide free
See all Prolactin/PRL primary antibodies -
Description
Rabbit monoclonal [EPR19372] to Prolactin/PRL - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250714 is the carrier-free version of ab183968. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250714 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19372 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Prolactin/PRL antibody [EPR19372] (ab183968) at 1/5000 dilution
Lane 3 : Mouse uterus tissue lysate
Lane 4 : Rat kidney tissue lysate
Lane 5 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaThis data was developed using ab183968, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 8 seconds; Lanes 2-6: 3 second.
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This data was developed using ab183968, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pituitary tissue labeling Prolactin/PRL with ab183968 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed cytoplasmic staining on mouse anterior pituitary. The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
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This data was developed using ab183968, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling Prolactin/PRL with ab183968 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed negative staining on mouse kidney. The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
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This data was developed using ab183968, the same antibody clone in a different buffer formulation.Prolactin/PRL was immunoprecipitated from 0.35 mg of mouse pituitary whole cell lysate with ab183968 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183968 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: Mouse pituitary whole cell lysate, 10µg (Input). Lane 2: ab183968 IP in Mouse pituitary whole cell lysate. Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab183968 in Mouse pituitary whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds.
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