Rat Prolactin ELISA Kit (ab272780)
Key features and details
- Range: 2 ng/ml - 1000 ng/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Rat
Overview
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Product name
Rat Prolactin ELISA Kit
See all Prolactin/PRL kits -
Detection method
Colorimetric -
Sample type
Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Quantitative -
Range
2 ng/ml - 1000 ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat Prolactin ELISA Kit (ab272780) is intended for the quantitative determination of prolactin antigen in rat plasma.
Rat prolactin will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, anti-rat prolactin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate is used for color development which is measured at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rat prolactin. Color development is proportional to the concentration of prolactin in the samples.
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Tested applications
Suitable for: ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer 1 x 50ml Anti-Rabbit Streptavidin-HRP Secondary Reagent 1 vial Anti-Rat Prolactin Primary Antibody Lyophilized Vial 1 vial Rat Prolactin ELISA Plate 1 unit Rat Prolactin Standard Lyophilized Vial 1 vial TMB Substrate 1 x 10ml -
Research areas
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Relevance
Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands. -
Cellular localization
Secreted -
Alternative names
- Decidual prolactin
- GHA1
- Growth hormone A1
see all -
Database links
- Entrez Gene: 24683 Rat
- SwissProt: P01237 Rat
- Unigene: 9759 Rat
Images
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Example data
A typical standard curve. Example only.