Rat Prolactin ELISA Kit (ab272780) is intended for the quantitative determination of prolactin antigen in rat plasma.

Rat prolactin will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, anti-rat prolactin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate is used for color development which is measured at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rat prolactin. Color development is proportional to the concentration of prolactin in the samples.

• Signal Transduction
• Growth Factors/Hormones
• Hormones