Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (ab243564)
![Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (ab243564)](https://www.abcam.com/ps/products/243/ab243564/Images/ab243564-1-241064ELISA1.jpg "Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (ab243564)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPPTX-R7-94-2] to Estrone - BSA and Azide free
- Suitable for: Competitive ELISA
- Reacts with: Human
Overview
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Product name
Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free
See all Estrone primary antibodies -
Description
Rabbit monoclonal [EPPTX-R7-94-2] to Estrone - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Competitive ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Chemical/ Small Molecule corresponding to Estrone. (Estrone-6-CMO-BSA).
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General notes
ab243564 is the carrier-free version of ab241064. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab243564 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPPTX-R7-94-2 -
Isotype
IgG -
Research areas
Images
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Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (ab243564)
Goat anti-rabbit IgG was coated onto 96-wells. Serial dilution of Estrone-6 (0, 15, 30, 100, 250, 500, 1000, 2000pg/mL) was added (25μL). This was followed by adding of 50μL of HRP-conjugated Estrone and 2.0-2.5ng/mL ab241064 (50μL) into each well. Plates were incubated for 2 hours at room temperature by shaking. After washing, 125μL of Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. Then 125μL of stopping solution (acid solution) was dispensed and OD was read at 450nm on a microplate reader within 5 minutes.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241064). -
![Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (ab243564)](https://www.abcam.com/ps/products/243/ab243564/Images/ab243564-2-241064ELISA2.jpg)
Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (ab243564)ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] (ab241064) at 2.0 ng/ml.
ab241064 is specific to Estrone-6 and the percent cross reactivity with other compounds is demonstrated in the table.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241064). -
![Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (ab243564)](https://www.abcam.com/ps/products/243/ab243564/Images/ab243564-5-benefits-of-recombinant-antibodies.png)
Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (ab243564)
