Anti-Estrone antibody [EPPTX-R7-94-2] (ab241064)
![Anti-Estrone antibody [EPPTX-R7-94-2] (ab241064)](https://www.abcam.com/ps/products/241/ab241064/Images/ab241064-1-241064ELISA1.jpg "Anti-Estrone antibody [EPPTX-R7-94-2] (ab241064)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPPTX-R7-94-2] to Estrone
- Suitable for: Competitive ELISA
- Reacts with: Human
Overview
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Product name
Anti-Estrone antibody [EPPTX-R7-94-2]
See all Estrone primary antibodies -
Description
Rabbit monoclonal [EPPTX-R7-94-2] to Estrone -
Host species
Rabbit -
Tested applications
Suitable for: Competitive ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Chemical/ Small Molecule corresponding to Estrone. (Estrone-6-CMO-BSA).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPPTX-R7-94-2 -
Isotype
IgG -
Research areas
Images
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Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] (ab241064)
Goat anti-rabbit IgG was coated onto 96-wells. Serial dilution of Estrone-6 (0, 15, 30, 100, 250, 500, 1000, 2000pg/mL) was added (25μL). This was followed by adding of 50μL of HRP-conjugated Estrone and 2.0-2.5ng/mL ab241064 (50μL) into each well. Plates were incubated for 2 hours at room temperature by shaking. After washing, 125μL of Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. Then 125 μL of stopping solution (acid solution) was dispensed and OD was read at 450nm on a microplate reader within 5 minutes.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
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![Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] (ab241064)](https://www.abcam.com/ps/products/241/ab241064/Images/ab241064-2-241064ELISA2.jpg)
Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] (ab241064)ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] (ab241064) at 2.0 ng/ml.
ab241064 is specific to Estrone-6 and the percent cross reactivity with other compounds is demonstrated in the table.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
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![Anti-Estrone antibody [EPPTX-R7-94-2] (ab241064)](https://www.abcam.com/ps/products/241/ab241064/Images/ab241064-3-benefits-of-recombinant-antibodies.png)
Anti-Estrone antibody [EPPTX-R7-94-2] (ab241064)
