Mouse Leptin R ELISA Kit (ab267584)
Key features and details
- Sensitivity: 32 pg/ml
- Range: 32.77 pg/ml - 8000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Leptin R ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
32 pg/ml -
Range
32.77 pg/ml - 8000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 145.1 139% - 149% Plasma 142.6 124% - 149% Cell culture media 137 128% - 147% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Leptin R ELISA Kit is designed for the quantitative determination of Leptin R in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for mouse Leptin R coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin R present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Leptin R antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin R bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse 4-1BB (TNFRSF9/CD137), Ace, ALK-1, Cardiotrophin-1 (CT-1), CD27 (TNFRSF7), CD32a, CD40 Ligand (TNFSF5), CTLA-4 (CD152), Decorin, DKK-1, Dtk, Endoglin (CD105), Flt-3 Ligand, Galectin-1, Galectin-3, Gas 1, Gas 6, GITR Ligand (TNFSF18), HAI-1, HGFR, IL-1 R4 (ST2), IL-3 R beta, IL-9, JAM-A (CD321/F11R), L-Selectin (CD62L), Lymphotactin (XCL1), MAdCAM-1, MFG-E8, MIP-3 beta (CCL19), Neprilysin, Pentraxin-3 (TSG-14), RAGE, TACI (TNFRSF13B), TREM-1, TROY (TNFRSF19), TSLP, TWEAK R (TNFRSF12), VEGFR1, VEGFR3.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Mouse Leptin R coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Mouse Leptin R Detection Antibody 2 vials Mouse Leptin R Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway. -
Tissue specificity
Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 4 fibronectin type-III domains.
Contains 1 Ig-like (immunoglobulin-like) domain. -
Domain
The cytoplasmic domain may be essential for intracellular signal transduction by activation of JAK tyrosine kinase and STATs.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Post-translational
modificationsOn ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation and, for interaction with SOCS3 (By similarity). Phosphorylation on Tyr-1141 is required for STAT3 binding/activation. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- CD 295
- CD295
- CD295 antigen
see all -
Database links
- Entrez Gene: 16847 Mouse
- SwissProt: P48356 Mouse
- Unigene: 259282 Mouse
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.