Anti-EG-VEGF antibody [EPR9699] (ab150375)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9699] to EG-VEGF
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human
Overview
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Product name
Anti-EG-VEGF antibody [EPR9699]
See all EG-VEGF primary antibodies -
Description
Rabbit monoclonal [EPR9699] to EG-VEGF -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseWB Mouse -
Immunogen
Synthetic peptide within Human EG-VEGF aa 50-150 (C terminal). The exact sequence is proprietary.
(Peptide available asab206122) -
Positive control
- Raw 264.7, NIH/3T3, and ECV-304 cell lysates; NIH/3T3 cells.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9699 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-EG-VEGF antibody [EPR9699] (ab150375) at 1/1000 dilution
Lane 1 : Raw 264.7 cell lysate.
Lane 2 : NIH/3T3 cell lysate.
Lane 3 : ECV-304 cell lysate.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 12 kDa
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Flow cytometric analysis of permeabilized NIH/3T3 cells labelling EG-VEGF with ab150375 at 1/10 dilution (red) or a Rabbit IgG (negative, green).
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