Anti-p53 antibody (ab31333)
Key features and details
- Rabbit polyclonal to p53
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-p53 antibody
See all p53 primary antibodies -
Description
Rabbit polyclonal to p53 -
Host species
Rabbit -
Specificity
ab31333 detects endogenous levels of p53 protein around Serine 6. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human p53 (N terminal). The antiserum was produced against synthesized non-phosphopeptide derived from human p53 around the phosphorylation site of serine 6.
Database link: P04637
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
This antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-p53 antibody (ab31333)All lanes : Anti-p53 antibody (ab31333) at 1/1000 dilution
Lane 1 : MDA-MB-435 cell extract treated with UV
Lane 2 : 293 cell extract treated with UV
Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody (ab31333)ab31333, at a dilution of 1/100, staining p53 in paraffin embedded human breast carcinoma by Immunohistochemistry.
Left: untreated.
Right: treated with synthesized peptide.
