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Anti-Bax antibody [5B7] (ab3191)

Price and availability

321 638 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-Bax antibody [5B7] (ab3191)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [5B7] to Bax
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Mouse

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Overview

  • Product name

    Anti-Bax antibody [5B7]
    See all Bax primary antibodies
  • Description

    Mouse monoclonal [5B7] to Bax
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    IP
    Mouse
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: A20 and C2C12 lysate. Flow Cyt: RAW 264.7 cells.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.5% BSA, PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    5B7
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Bcl 2 family
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Apoptosis marker and proteins ELISA kits
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Bcl 2 family
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Images

  • Western blot - Anti-Bax antibody [5B7] (ab3191)
    Western blot - Anti-Bax antibody [5B7] (ab3191)
    Lane 1 : Anti-Bax antibody [5B7] (ab3191) at 0.417 mg/ml
    Lane 2 : Anti-Bax antibody [5B7] (ab3191) at 0.417 µg/ml

    Lane 1 : A20 (Mouse reticulum sarcoma B lymphocyte) lysate
    Lane 2 : C2C12 (Mouse myoblasts) lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 21 kDa


    Exposure time: 26 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-Bax antibody [5B7] (ab3191)
    Flow Cytometry - Anti-Bax antibody [5B7] (ab3191)

    Flow Cytometry analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling Bax with ab3191 at a 1.043µg/ml  (red) compared to a mouse monoclonal IgG isotype control (black) and cells without incubation with primary antibody and secondary antibody (blue). Goat anti-mouse IgG (Alexa Fluor® 488, ab150113) was used as the secondary at a 1/2000 dilution.

  • Immunoprecipitation - Anti-Bax antibody [5B7] (ab3191)
    Immunoprecipitation - Anti-Bax antibody [5B7] (ab3191)

    Bax was immunoprecipitated from RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab190908 at 13.9µg/ml. Western blot was performed from the immunoprecipitate using ab3191 at 1/1000 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used as secondary antibody at 1/50000 dilution.
    Lane 1: RAW 264.7 whole cell lysate 10ug (Input).
    Lane 2: ab3191 IP in RAW 264.7 whole cell lysate.
    Lane 3: Mouse monoclonal IgG instead of ab3191 in RAW 264.7 whole cell lysate.
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 30 seconds.

  • Anti-Bax antibody [5B7] (ab3191)
    Anti-Bax antibody [5B7] (ab3191)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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