Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bax antibody [5B7] - BSA and Azide free (ab252258)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [5B7] to Bax - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Bax antibody [5B7] - BSA and Azide free
    See all Bax primary antibodies

  • Description

    Mouse monoclonal [5B7] to Bax - BSA and Azide free

  • Host species

    Mouse

  • Specificity

    Antibody raised against a synthetic peptide that is not shared by rat or human Bax.

  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A20 and C2C12 lysate. Flow Cyt: RAW 264.7 cells.

  • General notes

    ab252258 is the PBS only version of ab3191.

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Bax antibody [5B7] - BSA and Azide free (ab252258)
    Western blot - Anti-Bax antibody [5B7] - BSA and Azide free (ab252258)
    All lanes : Anti-Bax antibody [5B7] (ab3191) at 1/1000 dilution

    Lane 1 : A20 (Mouse reticulum sarcoma B lymphocyte) lysate
    Lane 2 : C2C12 (Mouse myoblasts) lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 21 kDa


    Exposure time: 26 seconds


    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab3191).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-Bax antibody [5B7] - BSA and Azide free (ab252258)
    Flow Cytometry - Anti-Bax antibody [5B7] - BSA and Azide free (ab252258)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab3191).

    Flow Cytometry analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling Bax with ab3191 at 1.043µg/ml (red) compared to a mouse monoclonal IgG isotype control (black) and cells without incubation with primary antibody and secondary antibody (blue). Goat anti-mouse IgG (Alexa Fluor® 488, ab150113) was used as the secondary at a 1/2000 dilution.

  • Immunoprecipitation - Anti-Bax antibody [5B7] - BSA and Azide free (ab252258)
    Immunoprecipitation - Anti-Bax antibody [5B7] - BSA and Azide free (ab252258)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab3191).

    Bax was immunoprecipitated from RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab190908 at 13.9µg/ml . Western blot was performed from the immunoprecipitate using ab3191 at 1/1000 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used as secondary antibody at 1/50000 dilution.
    Lane 1: RAW 264.7 whole cell lysate 10ug (Input).
    Lane 2: ab3191 IP in RAW 264.7 whole cell lysate.
    Lane 3: Mouse monoclonal IgG instead of ab3191 in RAW 264.7 whole cell lysate.
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 30 seconds.

  • Anti-Bax antibody [5B7] - BSA and Azide free (ab252258)
    Anti-Bax antibody [5B7] - BSA and Azide free (ab252258)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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